Wang Yuqiong, Liao Yangci, Gou Changlong, Zhang Hang, Chen Liming, Bao Yuhong
College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, Inner Mongolia, China.
Institute of Pratacultural, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa, Tibet, China.
Front Microbiol. 2024 May 22;15:1365254. doi: 10.3389/fmicb.2024.1365254. eCollection 2024.
The efficient utilization of straw resources as animal feed has gained considerable attention. The objective of this study was to evaluate whether treatment alters the chemical composition and antioxidant activity of highland barley straw and enhances its functional value as a ruminant feed.
The chemical composition, antioxidant capacity, and metabolomic profile of highland barley straw were determined after 21 days of solid-state fermentation with at 25°C. The differential metabolites between fermented and unfermented highland barley straw were identified by LC-MS and the relationship between the identified metabolites and antioxidant capacity was elucidated.
The results showed that, compared with untreated highland barley straw, the crude protein and ether extract contents were higher (51.55 and 76.43%, respectively) in highland barley straw after 21 days of incubation with , whereas the hemicellulose, cellulose, and acid detergent lignin contents were lower (2.48, 25.08, and 45%, respectively). The total antioxidant capacity was significantly higher in -treated than in untreated highland barley straw. In total, 600 differential metabolites (301 upregulated and 299 downregulated) were identified between -fermented and unfermented highland barley straw. Correlation analysis results showed that Fe scavenging and total phenolic content were strongly correlated with total antioxidant capacity. Meanwhile, the differential flavonoid metabolites between fermented and unfermented highland barley straw were primarily associated with antioxidant activity, with kaempferol 3-xylosylglucoside, isoginkgetin, and rhoifolin being the most representative.
Thus, this study demonstrates that could enhance the functional value of highland barley straw, showing the potential of for improving the utilization of agricultural straws in ruminants.
秸秆资源作为动物饲料的高效利用已受到广泛关注。本研究的目的是评估[具体处理方式]处理是否会改变青稞秸秆的化学成分和抗氧化活性，并提高其作为反刍动物饲料的功能价值。
在25℃下对青稞秸秆进行21天固态发酵后，测定其化学成分、抗氧化能力和代谢组学特征。通过液相色谱-质谱联用（LC-MS）鉴定发酵和未发酵青稞秸秆之间的差异代谢物，并阐明所鉴定代谢物与抗氧化能力之间的关系。
结果表明，与未处理的青稞秸秆相比，经[具体处理方式]处理21天的青稞秸秆粗蛋白和乙醚提取物含量更高（分别为51.55%和76.43%），而半纤维素、纤维素和酸性洗涤木质素含量更低（分别为2.48%、25.08%和45%）。经[具体处理方式]处理的青稞秸秆总抗氧化能力显著高于未处理的。总共在经[具体处理方式]发酵和未发酵的青稞秸秆之间鉴定出600种差异代谢物（301种上调和299种下调）。相关性分析结果表明，铁清除能力和总酚含量与总抗氧化能力密切相关。同时，发酵和未发酵青稞秸秆之间的差异黄酮类代谢物主要与抗氧化活性相关，山奈酚3-木糖基葡萄糖苷、异银杏双黄酮和芸香柚皮苷是最具代表性的。
因此，本研究表明[具体处理方式]可以提高青稞秸秆的功能价值，显示出[具体处理方式]在提高反刍动物对农业秸秆利用率方面的潜力。
