Gupta Aditi, Sharma Satyawati, Kumar Ashwani, Alam Pravej, Ahmad Parvaiz
Department of Chemistry, St. Stephen's College, Delhi UniversityDelhi, India; Centre for Rural Development and Technology, Indian Institute of Technology DelhiNew Delhi, India.
Centre for Rural Development and Technology, Indian Institute of Technology Delhi New Delhi, India.
Front Microbiol. 2016 Oct 13;7:1529. doi: 10.3389/fmicb.2016.01529. eCollection 2016.
Residual biogas slurries (BGS) of detoxified mahua cake and cow dung were used as supplements to enhance the yield and nutritional quality of on wheat straw (WS). Supplementation with 20% BGS gave a maximum yield of 1155 gkg fruit bodies, furnishing an increase of 95.1% over WS control. Significant increase ( ≤ 0.05) in protein content (29.6-38.9%), sugars (29.1-32.3%) and minerals (N, P, K, Fe, Zn) was observed in the fruit bodies. Principle component analysis (PCA) was performed to see the pattern of correlation within a set of observed variables and how these different variables varied in different treatments. PC1 and PC2 represented 90% of total variation in the observed variables. Moisture (%), lignin (%), celluloses (%), and C/N ratio were closely correlated in comparison to Fe, N, and saponins. PCA of amino acids revealed that, PC1 and PC2 represented 74% of total variation in the data set. HPLC confirmed the absence of any saponin residues (characteristic toxins of mahua cake) in fruit bodies and mushroom spent. FTIR studies showed significant degradation of celluloses (22.2-32.4%), hemicelluloses (14.1-23.1%) and lignin (27.4-39.23%) in the spent, along with an increase in nutrition content. The study provided a simple, cost effective approach to improve the yield and nutritional quality of by resourceful utilization of BGS.
解毒后的麻疯树饼粕和牛粪的残余沼液(BGS)被用作补充剂,以提高平菇在小麦秸秆(WS)上的产量和营养品质。添加20%的BGS时,子实体产量最高可达1155 g/kg,比WS对照增加了95.1%。在子实体中观察到蛋白质含量(29.6 - 38.9%)、糖类(29.1 - 32.3%)和矿物质(氮、磷、钾、铁、锌)显著增加(P≤0.05)。进行主成分分析(PCA)以观察一组观测变量之间的相关模式,以及这些不同变量在不同处理中的变化情况。PC1和PC2代表了观测变量总变异的90%。与铁、氮和皂苷相比,水分(%)、木质素(%)、纤维素(%)和碳氮比密切相关。氨基酸的PCA分析表明,PC1和PC2代表了数据集中总变异的74%。高效液相色谱法(HPLC)证实子实体和废菌料中不存在任何皂苷残留(麻疯树饼粕的特征毒素)。傅里叶变换红外光谱(FTIR)研究表明,废菌料中的纤维素(22.2 - 32.4%)、半纤维素(14.1 - 23.1%)和木质素(27.4 - 39.23%)有显著降解,同时营养成分增加。该研究提供了一种简单、经济有效的方法,通过巧妙利用BGS来提高平菇的产量和营养品质。