含大鼠催乳素结构基因的重组DNA的构建与分析
Construction and analysis of recombinant DNAs containing a structural gene for rat prolactin.
作者信息
Gubbins E J, Maurer R A, Hartley J L, Donelson J E
出版信息
Nucleic Acids Res. 1979 Mar;6(3):915-30. doi: 10.1093/nar/6.3.915.
Poly A-containing RNA enriched in prolactin-coding sequences was isolated from female rate pituitaries after induction with diethylstilbesterol. Double stranded cDNA was synthesized from this RNA and inserted into plasmid pBR322 at the Pst I site via the poly(dG):polyy(dC) tailing method. E. coli was transformed with this DNA and the recombinant plasmid in one of the transformants characterized in detail. About half of its 900 base pair cDNA insert was sequenced. The DNA sequence is consistent with most of the reported amino acid sequence of rat preprolactin. In addition, the recombinant plasmids in two of the other transformants appear to contain growth hormone coding sequences.
在用己烯雌酚诱导后的雌性大鼠垂体中分离出富含催乳素编码序列的含聚腺苷酸RNA。从该RNA合成双链cDNA，并通过聚（dG）：聚（dC）加尾法将其插入质粒pBR322的Pst I位点。用该DNA转化大肠杆菌，并对其中一个转化体中的重组质粒进行了详细表征。对其900个碱基对的cDNA插入片段约一半进行了测序。该DNA序列与报道的大鼠前催乳素的大多数氨基酸序列一致。此外，其他两个转化体中的重组质粒似乎含有生长激素编码序列。
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