大鼠胰岛素基因：包含编码序列的质粒构建
Rat insulin genes: construction of plasmids containing the coding sequences.
作者信息
Ullrich A, Shine J, Chirgwin J, Pictet R, Tischer E, Rutter W J, Goodman H M
出版信息
Science. 1977 Jun 17;196(4296):1313-9. doi: 10.1126/science.325648.
PMID:325648
Abstract
Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated 3' terminal region of the mRNA. A fourth plasmid contains sequences derived from the A chain region of rat preproinsulin II.
摘要
已经构建了重组细菌质粒，其包含从大鼠胰岛信使核糖核酸制备的互补脱氧核糖核酸。三种质粒含有克隆序列，分别代表大鼠胰岛素原I的完整编码区、胰岛素原I前肽的一部分以及信使核糖核酸的非翻译3'末端区域。第四种质粒含有源自大鼠胰岛素原II A链区域的序列。
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