Singer R A, Johnston G C
Exp Cell Res. 1985 Apr;157(2):387-96. doi: 10.1016/0014-4827(85)90124-7.
Cells of the yeast S. cerevisiae can be cultured under conditions in which the DNA-division sequence, and not cellular growth, is the rate-limiting feature for cell proliferation. Relief of these limiting conditions, which has been shown to allow accelerated cell division, did not result in increased rates of cell mass accumulation during the time of rapid cell division. Moreover, under conditions of constant DNA-division sequence constraint, populations of smaller cells produced by slowing growth with cycloheximide gave rise to large cells when cycloheximide was removed. These observations suggest that in proliferating cells of S. cerevisiae the DNA-division sequences does not affect cellular growth.
即DNA分裂序列而非细胞生长是细胞增殖的限速因素。已证明解除这些限制条件可使细胞分裂加速，但在快速细胞分裂期间，细胞质量积累速率并未增加。此外，在DNA分裂序列持续受限的条件下，用环己酰亚胺减缓生长产生的较小细胞群体，在去除环己酰亚胺后会产生较大细胞。这些观察结果表明，在酿酒酵母的增殖细胞中，DNA分裂序列不影响细胞生长。
