College of Agriculture, Ibaraki University, Ibaraki 300-0393, Japan; United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
College of Agriculture, Ibaraki University, Ibaraki 300-0393, Japan.
J Biotechnol. 2024 Aug 10;391:64-71. doi: 10.1016/j.jbiotec.2024.06.003. Epub 2024 Jun 4.
We investigated suitable culture conditions for the production of the blue pigment phycocyanin (PC) from the unique filamentous cyanobacteria Pseudanabaena sp. ABRG5-3 and Limnothrix sp. SK1-2-1. White, green, or red LED irradiation at 30 μmol photons/m/s was effective for phycocyanin production when compared with Arthrospira platensis (Spirulina) sp. NIES-39, which is generally grown under high light irradiation. To investigate the safety of the cyanobacteria, ABRG5-3 cells were subjected to Ames (reverse mutation) tests and single oral-dose rat studies, which revealed non-mutagenic and non-toxic properties. When three purified phycocyanins (abPC, skPC, and spPC) were subjected to agarose gel electrophoresis, they showed different mobility, indicating that each phycocyanin has unique properties. abPC exhibited strong antiglycation activities as novel function.
我们研究了从独特的丝状蓝藻 Pseudanabaena sp. ABRG5-3 和 Limnothrix sp. SK1-2-1 生产蓝色色素藻蓝蛋白 (PC) 的适宜培养条件。与通常在高光照射下生长的 Arthrospira platensis (Spirulina) sp. NIES-39 相比，白色、绿色或红色 LED 以 30 μmol 光子/m/s 的光强照射对藻蓝蛋白的生产更有效。为了研究蓝藻的安全性，将 ABRG5-3 细胞进行了艾姆斯（反向突变）试验和单次口服大鼠研究，结果表明其没有致突变性和毒性。当对三种纯化的藻蓝蛋白（abPC、skPC 和 spPC）进行琼脂糖凝胶电泳时，它们显示出不同的迁移率，表明每种藻蓝蛋白都具有独特的性质。abPC 表现出强烈的抗糖化活性，具有新的功能。
