College of Agriculture, Ibaraki University, 3-21-1 Ami, Ibaraki 300-0393, Japan; United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho Fuchu-shi, Tokyo 183-8509, Japan.
College of Agriculture, Ibaraki University, 3-21-1 Ami, Ibaraki 300-0393, Japan; United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho Fuchu-shi, Tokyo 183-8509, Japan.
J Biotechnol. 2024 Nov 20;395:180-188. doi: 10.1016/j.jbiotec.2024.08.006. Epub 2024 Aug 15.
As current methods of production of phycocyanobilin, a photosynthetic blue pigment derived from phycocyanin of filamentous cyanobacteria, Pseudanabaena sp. ABRG5-3, Limnothrix sp. SK1-2-1, and Spirulina sp., exhibit a low extraction efficiency, a new extraction method using ethanol extraction as a type of solvolysis with an autoclave (130 ℃, 5.7 bar, 10 min) was developed in this study. This method exhibited high efficiency and enabled easy recovery of the three types of phycocyanobilins. The identity of the three types of phycocyanobilins was confirmed by high-performance liquid chromatography and electrospray ionization-tandem mass spectrometry. Phycocyanobilins were stable at high temperatures (80 ℃) and acidic (pH 3) conditions. Phycocyanobilins also possessed a remarkable antioxidant property. This is the first time that a simple phycocyanobilin extraction method with a recovery rate of more than 60 % and approximately 1 % per dry cell weight of filamentous cyanobacteria has been demonstrated. This novel production method is thus convenient and effective for obtaining high-purity phycocyanobilins.
目前从蓝藻门丝状蓝细菌钝顶螺旋藻、鱼腥藻 ABRG5-3 和席藻属 SK1-2-1 的藻蓝蛋白中提取光合作用蓝色色素藻蓝胆素的方法提取效率较低，本研究开发了一种新的提取方法，使用乙醇提取作为一种加压釜中的溶剂分解反应（130℃，5.7 bar，10 min）。该方法效率高，可轻松回收三种类型的藻蓝胆素。三种类型的藻蓝胆素通过高效液相色谱和电喷雾串联质谱法确认。藻蓝胆素在高温（80℃）和酸性（pH 3）条件下稳定。藻蓝胆素还具有显著的抗氧化特性。这是首次证明从丝状蓝藻中以超过 60%的回收率和每干细胞重量约 1%的比例提取藻蓝胆素的简单方法。因此，这种新型生产方法方便、有效地获得高纯度的藻蓝胆素。
