Medina Juan Gerardo Sánchez, Camacho José Luis Cuéllar, Ruiz Garcia Jaime, Mira Alex, Martínez Martínez Rita Elizabeth, Comas-García Mauricio, Rangel Arturo Garrocho, Pozos-Guillén Amaury, Romo Saray Aranda
Department of Microbiology, Faculty of Dentistry, Autonomous University of San Luis Potosi, Manuel Nava No. 2, Col Universitaria, 78290, San Luis Potosí, S.L.P., Mexico.
Faculty of Science, Autonomous University of San Luis Potosi, San Luis Potosí, S.L.P., Mexico.
Int Microbiol. 2025 Feb;28(2):289-298. doi: 10.1007/s10123-024-00525-7. Epub 2024 Jun 7.
Probiotic bacteria inhibit aggregation, biofilm formation, and dimorphism of Candida spp. However, the effects of a new probiotic, Streptococcus dentisani, on the growth of Candida albicans and Candida glabrata biofilms are unknown.
To determine the effect of S. dentisani on the different phases of C. albicans and C. glabrata biofilm development.
Growth quantification and ultrastructural analyses were performed on biofilms of C. albicans ATCC 90028, C. glabrata ATCC 2001, and clinical isolates of C. albicans from oral candidiasis (CA-C1), caries (CA-CR1), and periodontal pocket (CA-P1) treated with cell suspensions of S. dentisani CECT 7746. Cell viability was determined by quantifying colony-forming units (CFU/mL). The ultrastructural analyses were done with atomic force microscopy.
S. dentisani induced a significant reduction (p < 0.05) of CFU/mL of immature and mature biofilm in all strains of C. albicans and C. glabrata. Microscopic analysis revealed that S. dentisani reduced C. albicans density in mixed biofilm. The fungus-bacteria interaction affected cell membrane integrity in yeast.
For the first time, our data elucidate the antifungal effect of S. dentisani on the development of C. albicans and C. glabrata biofilms, supporting its usefulness as a niche-specific probiotic to prevent and treat oral dysbiosis.
益生菌可抑制念珠菌属的聚集、生物膜形成和二态性。然而，新型益生菌齿双歧链球菌对白色念珠菌和光滑念珠菌生物膜生长的影响尚不清楚。
确定齿双歧链球菌对白色念珠菌和光滑念珠菌生物膜不同发育阶段的影响。
对白色念珠菌ATCC 90028、光滑念珠菌ATCC 2001以及来自口腔念珠菌病（CA-C1）、龋齿（CA-CR1）和牙周袋（CA-P1）的白色念珠菌临床分离株的生物膜，用齿双歧链球菌CECT 7746细胞悬液进行处理，并进行生长定量和超微结构分析。通过定量菌落形成单位（CFU/mL）来确定细胞活力。用原子力显微镜进行超微结构分析。
齿双歧链球菌可使所有白色念珠菌和光滑念珠菌菌株的未成熟和成熟生物膜的CFU/mL显著降低（p < 0.05）。显微镜分析显示，齿双歧链球菌降低了混合生物膜中白色念珠菌的密度。真菌与细菌的相互作用影响了酵母细胞膜的完整性。
我们的数据首次阐明了齿双歧链球菌对白色念珠菌和光滑念珠菌生物膜发育的抗真菌作用，支持其作为一种特定生态位益生菌用于预防和治疗口腔生态失调的有效性。
