School of Life Sciences/Hebei Basic Science Center for Biotic Interaction, Hebei University, Baoding, 071002, China.
Institute of Life Science and Green Development, Hebei University, Baoding, 071002, China.
BMC Genomics. 2024 Jun 6;25(1):570. doi: 10.1186/s12864-024-10453-5.
Compound eyes formation in decapod crustaceans occurs after the nauplius stage. However, the key genes and regulatory mechanisms of compound eye development during crustacean embryonic development have not yet been clarified. In this study, RNA-seq was used to investigate the gene expression profiles of Neocaridina denticulata sinensis from nauplius to zoea stage. Based on RNA-seq data analysis, the phototransduction and insect hormone biosynthesis pathways were enriched, and molting-related neuropeptides were highly expressed. There was strong cell proliferation in the embryo prior to compound eye development. The formation of the visual system and the hormonal regulation of hatching were the dominant biological events during compound eye development. The functional analysis of DEGs across all four developmental stages showed that cuticle formation, muscle growth and the establishment of immune system occurred from nauplius to zoea stage. Key genes related to eye development were discovered, including those involved in the determination and differentiation of the eye field, eye-color formation, and visual signal transduction. In conclusion, the results increase the understanding of the molecular mechanism of eye formation in crustacean embryonic stage.
复眼在十足目甲壳动物的无节幼体期后形成。然而，甲壳动物胚胎发育过程中复眼发育的关键基因和调控机制尚不清楚。在这项研究中，我们使用 RNA-seq 技术研究了从无节幼体到溞状幼体阶段的中华锯齿米虾的基因表达谱。基于 RNA-seq 数据分析，富集了光转导和昆虫激素生物合成途径，蜕皮相关神经肽高度表达。在复眼发育之前，胚胎中有强烈的细胞增殖。视觉系统的形成和孵化的激素调节是复眼发育过程中的主导生物学事件。对四个发育阶段的 DEGs 的功能分析表明，从无节幼体到溞状幼体阶段发生了表皮形成、肌肉生长和免疫系统的建立。发现了与眼睛发育相关的关键基因，包括参与眼区确定和分化、眼睛颜色形成以及视觉信号转导的基因。总之，这些结果增加了我们对甲壳动物胚胎阶段眼睛形成的分子机制的理解。
