School of Life Sciences, Institute of Life Sciences and Green Development, Hebei University, Baoding, 071002, China.
School of Life Sciences, Institute of Life Sciences and Green Development, Hebei University, Baoding, 071002, China.
Fish Shellfish Immunol. 2022 Feb;121:23-30. doi: 10.1016/j.fsi.2021.10.029. Epub 2021 Nov 3.
Neocaridina denticulate sinensis is a promising crustacean model species due to its merits in raising and breeding. However, its molecular responses to copper remains largely unknown. In the present research, RNA-seq was used to mine the alteration in transcriptome of N. denticulate sinensis hepatopancreas under copper exposure. A total of 16,423 DEGs was identified between control and Cu treatment groups. GO enrichment analysis of all DEGs suggested down-regulated genes exceeded up-regulated genes in all the significantly enriched terms, except for RNA polymerase III complex (GO:0005666). KEGG analysis showed Cu exposure only induced two significantly enriched pathways, including Phagosome (ko04145) and Pathogenic Escherichia coli infection (ko05130). Besides, pattern recognition receptors as Toll, lectin B, CTL1 and SRB, AMPs as crustin type I, lysozyme, and NOS were down-regulated after Cu exposure, while hemocyanin, MT, HSP70 and HSP90 were significantly up-regulated, implying these molecules may play vital role in Cu detoxification of N. denticulate sinensis. Our results here provide research direction of heavy metal detoxification of N. denticulate sinensis, simultaneously enriched its genomic information.
中华锯齿米虾是一种很有前途的甲壳类模式生物，因为它在养殖方面有很多优点。然而，它对铜的分子反应在很大程度上仍然未知。在本研究中，使用 RNA-seq 技术来挖掘中华锯齿米虾在铜暴露下肝胰腺转录组的变化。在对照组和 Cu 处理组之间共鉴定出 16423 个差异表达基因。所有差异表达基因的 GO 富集分析表明，在所有显著富集的术语中，下调基因的数量超过了上调基因，除了 RNA 聚合酶 III 复合物（GO:0005666）。KEGG 分析表明，Cu 暴露仅诱导了两个显著富集的途径，包括吞噬体（ko04145）和致病性大肠杆菌感染（ko05130）。此外，铜暴露后，模式识别受体如 Toll、凝集素 B、CTL1 和 SRB、抗菌肽如甲壳质 I 型、溶菌酶和 NOS 下调，而血蓝蛋白、MT、HSP70 和 HSP90 显著上调，这表明这些分子可能在中华锯齿米虾的铜解毒中发挥重要作用。我们的研究结果为中华锯齿米虾的重金属解毒提供了研究方向，同时丰富了其基因组信息。
