School of Life Sciences/Hebei Basic Science Center for Biotic Interaction, Hebei University, Baoding 071002, China; Institute of Life Science and Green Development, Hebei University, Baoding 071002, China.
School of Life Sciences/Hebei Basic Science Center for Biotic Interaction, Hebei University, Baoding 071002, China.
Ecotoxicol Environ Saf. 2024 Oct 15;285:117072. doi: 10.1016/j.ecoenv.2024.117072. Epub 2024 Sep 19.
Trehalase plays an important role in insect metabolism and development by hydrolyzing blood sugar trehalose, but it seems to perform primarily an immunomodulatory function in crustaceans whose blood sugar is glucose. Metal ions as pollutants seriously affecting crustacean health, but studies on trehalase in metal immunity are still limited. In this study, a soluble trehalase (NdTre1) that could bind to multiple metals was identified from Neocaridina denticulata sinensis for investigating metal resistance. Expression profiling revealed that NdTre1 was mainly expressed in the gill and was significantly decreased following stimulation with copper (Cu²⁺) and cadmium (Cd²⁺). Transcriptomic analysis of gills revealed an increase in ecdysone synthesis after interference with NdTre1. Increased ecdysone activated the endogenous mitochondrial pathway and the mitogen activated protein kinase (MAPK) pathway to further induced apoptosis. In vitro, Escherichia coli overexpressing recombinant NdTre1 had higher survival and faster growth rates to better adapted the metal-containing medium. Overall, NdTre1 exercises an important immune function in shrimp resistance to metal stimulation by regulating apoptosis and molting. Further investigation can further explore specific response mechanisms of NdTre1 to multiple metals.
海藻糖酶在昆虫代谢和发育中起着重要作用，通过水解血糖海藻糖，但在血糖为葡萄糖的甲壳动物中，它似乎主要发挥免疫调节功能。金属离子作为污染物严重影响甲壳动物的健康，但关于金属免疫中的海藻糖酶的研究仍然有限。在这项研究中，从中华锯齿米虾中鉴定出一种可结合多种金属的可溶性海藻糖酶（NdTre1），用于研究金属抗性。表达谱分析表明，NdTre1 主要在鳃中表达，并在受到铜（Cu²⁺）和镉（Cd²⁺）刺激后显著减少。对鳃的转录组分析表明，干扰 NdTre1 后，蜕皮甾酮的合成增加。增加的蜕皮甾酮激活了内源性线粒体途径和丝裂原激活蛋白激酶（MAPK）途径，进一步诱导细胞凋亡。在体外，表达重组 NdTre1 的大肠杆菌具有更高的存活率和更快的生长速度，以更好地适应含金属的培养基。总的来说，NdTre1 通过调节细胞凋亡和蜕皮来行使虾类对金属刺激的重要免疫功能。进一步的研究可以进一步探索 NdTre1 对多种金属的具体反应机制。
