Hsieh P, Baum J
Invest Ophthalmol Vis Sci. 1985 Apr;26(4):457-63.
Extracellular matrices (ECM) isolated from chick embryo fibroblast and human and rabbit corneal stromal cells induce polarization and elongation of corneal endothelial cells in culture. ECM isolated from rabbit corneal or bovine aortic endothelial cells neither polarize nor elongate corneal endothelial cells in culture. By indirect immunofluorescence, fibronectin is seen as arrays of long fibers in fibroblastic ECM, whereas in endothelial ECM, fibronectin is found in discreet foci as short fibers. The morphology corneal endothelial cells in culture is associated with the structure of the ECM laid down; short fibers in clusters associated with a typical polygonal shape, long polarized fibers inducing a fibroblastic-like appearance. ECM isolated from both fibroblastic or endothelial sources promote cell growth. Since coating the plastic with plasma fibronectin or denatured collagen does not promote endothelial cell growth, the authors assume the polymerized fibronectin network or a component bound to this network enhances the growth of corneal endothelial cells cultured in the presence of serum.
从鸡胚成纤维细胞以及人和兔角膜基质细胞中分离出的细胞外基质（ECM）可诱导培养中的角膜内皮细胞极化和伸长。从兔角膜或牛主动脉内皮细胞中分离出的ECM在培养中既不会使角膜内皮细胞极化也不会使其伸长。通过间接免疫荧光法，在成纤维细胞ECM中，纤连蛋白呈现为长纤维阵列，而在内皮细胞ECM中，纤连蛋白以离散的焦点形式存在，呈短纤维状。培养中的角膜内皮细胞形态与所形成的ECM结构相关；簇状的短纤维与典型的多边形形状相关，长的极化纤维则诱导出成纤维细胞样外观。从成纤维细胞或内皮细胞来源分离出的ECM均可促进细胞生长。由于用血浆纤连蛋白或变性胶原蛋白包被塑料并不能促进内皮细胞生长，因此作者推测聚合的纤连蛋白网络或与此网络结合的一种成分可增强在血清存在下培养的角膜内皮细胞的生长。
