Fu Jing, You Longtai, Sun Daohan, Zhang Lu, Zhao Jingxia, Li Ping
Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing Institute of Traditional Chinese Medicine, Beijing, 100010, China.
Department of Pharmacy, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 100045, China.
Heliyon. 2024 May 23;10(11):e31909. doi: 10.1016/j.heliyon.2024.e31909. eCollection 2024 Jun 15.
Psoriasis is an inflammation-based skin illness marked by aggravated proliferation of epidermal cells. Shikonin is a natural naphthoquinone obtained from radix. It exerts anti-inflammatory and immunosuppressive effects. However, the poor water solubility and low bioavailability of shikonin limit its application. In this study, shikosin-loaded PLGA nanoparticle hydrogel was prepared and used to deliver the drug to the epidermis of psoriasis mice through local administration. The results demonstrated that shikosin-loaded PLGA nanoparticles inhibited HaCaT cell multiplication, increased drug uptake, and induced apoptosis of HaCaT cells. Results from Western blotting assays indicated that shikosin down-regulated the protein expressions of p65 and p-p65. Furthermore, shikonin mitigated psoriasis and decreased the concentrations of inflammation-inducing cytokines, i.e., IL17A, IL-17F, IL-22, IL-1β, and TNF-α. Taken together, these results suggest that shikonin-PLGA nanoparticles loaded in hydrogel system possess promising therapeutic potential for psoriasis.
银屑病是一种基于炎症的皮肤病，其特征为表皮细胞过度增殖。紫草素是从紫草中提取的一种天然萘醌。它具有抗炎和免疫抑制作用。然而，紫草素的水溶性差和生物利用度低限制了其应用。在本研究中，制备了负载紫草素的聚乳酸-羟基乙酸共聚物（PLGA）纳米颗粒水凝胶，并通过局部给药将药物递送至银屑病小鼠的表皮。结果表明，负载紫草素的PLGA纳米颗粒抑制了人永生化角质形成细胞（HaCaT细胞）的增殖，增加了药物摄取，并诱导了HaCaT细胞凋亡。蛋白质免疫印迹分析结果表明，紫草素下调了p65和磷酸化p65（p-p65）的蛋白表达。此外，紫草素减轻了银屑病症状，并降低了炎症诱导细胞因子白细胞介素17A（IL17A）、白细胞介素1（IL-1β）和肿瘤坏死因子-α（TNF-α）的浓度。综上所述，这些结果表明，负载在水凝胶系统中的紫草素-PLGA纳米颗粒对银屑病具有良好的治疗潜力。
