Li Tong, Chen Jing, Yang Gongqiang, Wu Yuqing, Zhou Juan, Xu Pengjun, Ren Feirong, Li Minghui, Wu Xujin, Qiao Gexia
Institute of Plant Protection/Henan Key Laboratory of Crop Pest Control/Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
J Agric Food Chem. 2024 Jun 7. doi: 10.1021/acs.jafc.4c02642.
L-type symbiont (SMLS) is a bacterial symbiont commonly found in the wheat aphid . A new aphid densovirus, densovirus (SmDV), was recently identified in . In this study, the similar cellular tropism of SmDV and SMLS in aphid embryos was uncovered using in situ hybridization. SmDV infection significantly decreased the longevity and number of offspring. However, the SmDV titers were significantly suppressed after SMLS transmission, thus reducing the negative effects of SmDV infection on fitness. Moreover, an integrative analysis of RNA-seq datasets showed that SMLS inhibited the expression of genes related to the phosphatidylinositol 3-kinase (Pl3K)/Akt pathways and further induced the expression of antiviral factors associated with the apoptosis and FoxO signaling pathways. These results indicate that SMLS mediates host antiviral defenses to inhibit the propagation of SmDV, which was further verified by an RNA interference assay.
L型共生菌（SMLS）是一种常见于麦蚜体内的细菌共生菌。最近在麦蚜中鉴定出一种新的蚜虫浓核病毒，即浓核病毒（SmDV）。在本研究中，通过原位杂交揭示了SmDV和SMLS在蚜虫胚胎中相似的细胞嗜性。SmDV感染显著降低了蚜虫的寿命和后代数量。然而，在SMLS传播后，SmDV滴度显著受到抑制，从而降低了SmDV感染对蚜虫适合度的负面影响。此外，RNA测序数据集的综合分析表明，SMLS抑制了与磷脂酰肌醇3激酶（Pl3K）/Akt信号通路相关基因的表达，并进一步诱导了与凋亡和FoxO信号通路相关的抗病毒因子的表达。这些结果表明，SMLS介导宿主抗病毒防御以抑制SmDV的传播，RNA干扰试验进一步验证了这一点。
