Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & National Health Commission Key Laboratory of Digital Technology of Stomatology, Beijing, China.
Department of General Dentistry II, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & National Health Commission Key Laboratory of Digital Technology of Stomatology, Beijing, China.
BMC Oral Health. 2024 Jun 7;24(1):667. doi: 10.1186/s12903-024-04415-2.
This study investigated the effect of carbodiimide (EDC) combined with Clearfil SE self-etch adhesive on the shear bond strength (SBS), crosslinking degree, denaturation temperature, and enzyme activity of dentin in vitro.
Collected human sound third molars were randomly divided into different groups with or without EDC treatment (0.01-1 M). The specimens (n = 16)were stored for 24 h (immediate) or 12 months (aging) before testing the SBS. Fine dentin powder was obtained and treated with the same solutions. Then the crosslinking degree, denaturation temperature (Td), and enzyme activity were tested. Statistical analysis was performed using a one-way analysis of variance (ANOVA) to compare the differences of data between groups (α = 0.05).
There was a significant drop in immediate SBS and more adhesive fracture of 1.0 M EDC group, while there were no significant differences among the other groups. SEM showed a homogeneous interface under all treatments. After 12 months of aging, the SBS significantly decreased. Less decreases of SBS in the 0.3 and 0.5 M groups were found. Due to thermal and enzymatical properties consideration, the 0.3 and 0.5 M treatments also showed higher cross-link degree and Td with lower enzyme activity.
0.3 and 0.5 M EDC may be favorable for delaying the aging of self-etch bond strength for 12 months. But it is still needed thoroughly study.
本研究旨在探讨碳化二亚胺（EDC）联合 Clearfil SE 自酸蚀黏结剂对体外牙本质剪切黏结强度（SBS）、交联度、变性温度和酶活性的影响。
收集的人第三磨牙随机分为有无 EDC 处理组（0.01-1 M）。将标本（n=16）储存 24 小时（即刻）或 12 个月（老化）后，测试 SBS。从牙本质粉中获得相同的溶液，并进行交联度、变性温度（Td）和酶活性测试。采用单因素方差分析（ANOVA）对组间数据的差异进行统计分析（α=0.05）。
1.0 M EDC 组即刻 SBS 显著下降，黏结剂断裂更多，而其他组间无显著差异。SEM 显示所有处理后的界面均匀。老化 12 个月后，SBS 显著下降。0.3 和 0.5 M 组 SBS 下降较少。考虑到热和酶学特性，0.3 和 0.5 M 处理也表现出较高的交联度和 Td，以及较低的酶活性。
0.3 和 0.5 M EDC 可能有利于延缓自酸蚀黏结强度 12 个月的老化。但仍需深入研究。
