桉树叶精油的化学成分及其组合作为治疗耳部细菌性感染的一种有前途的治疗方法的评估。

Chemical compositions of Eucalyptus sp. Essential oils and the evaluation of their combinations as a promising treatment against ear bacterial infections.

机构信息

Chemical, Pharmacological and Gallenic Development Laboratory, Faculty of Pharmacy, University of Monastir, Avenue Avicennne, 5019, Monastir, Tunisia.

Water, and Forestry. INRGREF. Laboratory of Management and Valorization of Forest Resources, University of Carthage, The National Research Institute of Rural Engineering, 2080, Ariana, Tunisia.

出版信息

BMC Complement Med Ther. 2024 Jun 7;24(1):220. doi: 10.1186/s12906-024-04494-2.

DOI:10.1186/s12906-024-04494-2

PMID:38849805

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11157906/

Abstract

BACKGROUND

The chemical composition and biological activities of Eucalyptus essential oils (EOs) have been documented in numerous studies against multiple infectious diseases. The antibacterial activity of individual Eucalyptus EOs against strains that cause ear infections was investigated in our previous study. The study's antibacterial activity was promising, which prompted us to explore this activity further with EO blends.

METHODS

We tested 15 combinations (9 binary combinations and 6 combinations of binary combinations) of Eucalyptus EOs extracted by hydrodistillation from eight Tunisian Eucalyptus species dried leaves against six bacterial strains responsible for ear infections: three bacterial isolates (Haemophilus influenzae, Haemophilus parainfluenzae, and Klebsiella pneumoniae) and three reference bacteria strains (Pseudomonas aeruginosa, ATTC 9027; Staphylococcus aureus, ATCC 6538; and Escherichia coli, ATCC 8739). The EOs were analyzed using GC/FID and GC/MS. The major compounds, as well as all values obtained from the bacterial growth inhibition assay, were utilized for statistical analysis.

RESULTS

The antibacterial activity of the EO blends exhibited significant variation within Eucalyptus species, bacterial strains, and the applied methods. Principal component analysis (PCA) and hierarchical cluster analysis (HCA), based on the diameters of the inhibition zone, facilitated the identification of two major groups and ten subgroups based on the level of antibacterial activity. The highest antibacterial activity was observed for the mixture of EOs extracted from E. panctata, E. accedens, and E. cladoclayx (paac) as well as E. panctata, E. wandoo, E. accedens, and E. cladoclayx (pwac) using the disc diffusion method. Additionally, significant activity was noted with EOs extracted from E. panctata, E. wandoo (pw) and E. panctata, E. accedens (pa) using the broth microdilution method.

CONCLUSION

Our findings suggest that certain EO combinations (paac, pwac, pw, and pa) could be considered as potential alternative treatment for ear infections due to their demonstrated highly promising antibacterial activities.

摘要

背景

桉树精油（EOs）的化学成分和生物活性已在众多针对多种传染病的研究中得到证实。在我们之前的研究中，已经研究了个别桉树 EO 对引起耳部感染的菌株的抗菌活性。该研究的抗菌活性很有前景，这促使我们进一步探索 EO 混合物的这种活性。

方法

我们测试了 15 种由 8 种来自突尼斯的桉树干叶通过水蒸馏提取的桉树 EO 的组合（9 种二元组合和 6 种二元组合的组合）对 6 种引起耳部感染的细菌菌株的抗菌活性：3 种细菌分离株（流感嗜血杆菌、副流感嗜血杆菌和肺炎克雷伯菌）和 3 种参考细菌菌株（铜绿假单胞菌，ATTC 9027；金黄色葡萄球菌，ATCC 6538；和大肠杆菌，ATCC 8739）。EOs 使用 GC/FID 和 GC/MS 进行分析。主要化合物以及从细菌生长抑制测定中获得的所有值均用于统计分析。

结果

EO 混合物的抗菌活性在桉树物种、细菌菌株和应用方法之间表现出显著差异。基于抑菌圈直径的主成分分析（PCA）和层次聚类分析（HCA）有助于根据抗菌活性水平识别出两个主要组和十个亚组。使用纸片扩散法，从 E. panctata、E. accedens 和 E. cladoclayx（paac）以及 E. panctata、E. wandoo、E. accedens 和 E. cladoclayx（pwac）提取的 EO 混合物表现出最高的抗菌活性。此外，使用肉汤微量稀释法从 E. panctata、E. wandoo（pw）和 E. panctata、E. accedens（pa）提取的 EO 也表现出显著的活性。