Laboratory of Applied Organic Chemistry, Faculty of Sciences and Techniques Sidi Mohamed Ben Abdellah University, Fez, Morocco; Laboratory of Functional Ecology and Environment, Faculty of Sciences and Techniques Sidi Mohamed Ben Abdellah University, Fez, Morocco.
Laboratory of Microbial Biotechnology, Faculty of Sciences and Techniques Sidi Mohamed Ben Abdellah University, Fez, Morocco.
Eur J Pharm Biopharm. 2018 May;126:211-220. doi: 10.1016/j.ejpb.2017.06.002. Epub 2017 Jun 3.
To increase the sensibility of Salmonella typhimurium strain, a mixture of Thymus vulgaris L. (T. vulgaris L.), Rosmarinus officinalis L. (R. officinalis L.) and Myrtus communis L. (M. communis L.) essential oils (EOs) was used in combined treatment by experimental design methodology (mixture design). The chemical composition of EOs was firstly identified by GC and GC/MS and their antibacterial activity was evaluated. The results of this first step have shown that thymol and borneol were the major compounds in T. vulgaris and M. communis L. EOs, respectively, while 1,8-cineole and α-pinene were found as major compounds in R. officinalis L. The same results have shown a strong antibacterial activity of T. vulgaris L. EO followed by an important power of M. communis L. EO against a moderate activity of R. officinalis L. EO. Besides, 1/20 (v/v) was the concentration giving a strain response classified as sensitive. From this concentration, the mixture design was performed and analyzed. The optimization of mixtures antibacterial activities has highlighted the synergistic effect between T. vulgaris L. and M. communis L. essential oils. A formulation comprising 55% of T. vulgaris L. and 45% of M. communis L. essential oils, respectively, can be considered for the increase of Salmonella typhimurium sensibility.
为了提高鼠伤寒沙门氏菌的敏感性，采用实验设计方法（混合设计），将百里香（Thymus vulgaris L.）、迷迭香（Rosmarinus officinalis L.）和桃金娘（Myrtus communis L.）精油混合进行联合处理。首先通过 GC 和 GC/MS 鉴定精油的化学成分，并评估其抗菌活性。这第一步的结果表明，百里香和桃金娘精油中的主要化合物分别为麝香草酚和龙脑，而迷迭香精油中的主要化合物为 1,8-桉叶素和α-蒎烯。同样的结果表明，百里香精油具有很强的抗菌活性，桃金娘精油的抗菌活性次之，迷迭香精油的抗菌活性中等。此外，1/20（v/v）是使菌株反应分类为敏感的浓度。从该浓度开始，进行了混合设计并进行了分析。混合物抗菌活性的优化突出了百里香精油和桃金娘精油之间的协同作用。可以考虑使用包含 55%百里香精油和 45%桃金娘精油的配方来提高鼠伤寒沙门氏菌的敏感性。
