Laboratory of Management and Valorization of Forest Resources, National Institute of Researches on Rural Engineering, Water and Forests, Ariana 2080, Tunisia.
Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, Italy.
Molecules. 2023 Oct 21;28(20):7211. doi: 10.3390/molecules28207211.
This study was conducted to examine the chemical composition of the essential oils (EOs) from six Tunisian species and to evaluate their anti-enzymatic and antibiofilm activities. The EOs were obtained through hydro-distillation of dried leaves and subsequently analyzed using GC/MS. The main class of compounds was constituted by oxygenated monoterpenes, particularly prominent in (75.7%), (72.8%), and (67%). Anti-enzymatic activities against cholinesterases, α-amylase, and α-glucosidase were evaluated using spectrophotometric methods. Notably, the , and EOs displayed potent acetylcholinesterase (AChE) inhibition (IC: 0.25-0.60 mg/mL), with exhibiting lower activity (IC: 1.2 mg/mL). and showed remarkable α-amylase inhibition (IC: 0.88 mg/mL), while and significantly hindered α-glucosidase (IC < 30 mg/mL), distinguishing them from other EOs with limited effects. Additionally, the EOs were assessed for their anti-biofilm properties of Gram-positive ( and ) and Gram-negative (, and ) bacterial strains. The EO demonstrated the main antibiofilm effect against and with an inhibition > 80% at 10 mg/mL. These findings could represent a basis for possible further use of EOs in the treatment of human microbial infections and/or as a coadjutant in preventing and treating Alzheimer's disease and/or diabetes mellitus.
本研究旨在考察来自六个突尼斯物种的精油（EOs）的化学成分，并评估它们的抗酶和抗生物膜活性。EOs 通过对干燥叶片进行水蒸馏获得，随后使用 GC/MS 进行分析。主要化合物类别由含氧单萜组成，在 （75.7%）、 （72.8%）和 （67%）中尤为突出。使用分光光度法评估对乙酰胆碱酯酶、α-淀粉酶和α-葡萄糖苷酶的抗酶活性。值得注意的是， 、 和 精油对乙酰胆碱酯酶（AChE）具有很强的抑制作用（IC：0.25-0.60 mg/mL），而 表现出较低的活性（IC：1.2 mg/mL）。 、 和 对α-淀粉酶有显著的抑制作用（IC：0.88 mg/mL），而 、 则显著抑制α-葡萄糖苷酶（IC<30 mg/mL），与其他作用有限的精油不同。此外，还评估了 EOs 对革兰氏阳性（ 和 ）和革兰氏阴性（ 、 和 ）细菌菌株的抗生物膜特性。 精油对 和 表现出主要的抗生物膜作用，在 10 mg/mL 时抑制率>80%。这些发现可能为进一步将 精油用于治疗人类微生物感染以及/或作为辅助治疗阿尔茨海默病和/或糖尿病的药物提供依据。
