Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Otolaryngology-Head and Neck Surgery, Kindai University School of Medicine, Osaka, Japan.
Department of Otolaryngology - Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
STAR Protoc. 2024 Jun 21;5(2):103118. doi: 10.1016/j.xpro.2024.103118. Epub 2024 Jun 8.
The avian inner ear can naturally regenerate sensory hair cells and is therefore an ideal candidate for investigating mechanisms leading to hair cell regeneration and functional recovery. Here, we present a surgical protocol for eliminating auditory hair cells via sisomicin injection into the lateral semicircular canal. We describe steps for multiplex mRNA detection in chicken basilar papilla and utricle sections. We then detail procedures for integrating immunohistochemistry for concurrent mRNA and protein visualization, complemented by S-phase labeling with EdU. For complete details on the use and execution of this protocol, please refer to Benkafadar et al., Benkafadar et al., Sato et al., Janesick et al., Scheibinger et al..
禽类内耳可以自然再生感觉毛细胞，因此是研究导致毛细胞再生和功能恢复的机制的理想候选者。在这里，我们通过向侧半规管注射西索米星来介绍一种消除听觉毛细胞的手术方案。我们描述了在鸡耳蜗基底乳头和椭圆囊切片中进行多路 mRNA 检测的步骤。然后，我们详细介绍了将免疫组织化学与同时进行的 mRNA 和蛋白质可视化相结合的程序，并用 EdU 进行 S 期标记进行补充。有关此方案的使用和执行的完整详细信息，请参阅 Benkafadar 等人、Benkafadar 等人、Sato 等人、Janesick 等人、Scheibinger 等人。
