Department of Otolaryngology - Head & Neck Surgery, Institute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Palo Alto, CA 94305, USA.
Department of Otolaryngology - Head & Neck Surgery, Institute for Stem Cell Biology & Regenerative Medicine, Stanford University School of Medicine, Palo Alto, CA 94305, USA.
Cell Rep. 2021 Mar 23;34(12):108900. doi: 10.1016/j.celrep.2021.108900.
In contrast to mammals, birds recover naturally from acquired hearing loss, which makes them an ideal model for inner ear regeneration research. Here, we present a validated single-cell RNA sequencing resource of the avian cochlea. We describe specific markers for three distinct types of sensory hair cells, including a previously unknown subgroup, which we call superior tall hair cells. We identify markers for the supporting cells associated with tall hair cells, which represent the facultative stem cells of the avian inner ear. Likewise, we present markers for supporting cells that are located below the short cochlear hair cells. We further infer spatial expression gradients of hair cell genes along the tonotopic axis of the cochlea. This resource advances neurobiology, comparative biology, and regenerative medicine by providing a basis for comparative studies with non-regenerating mammalian cochleae and for longitudinal studies of the regenerating avian cochlea.
与哺乳动物不同，鸟类可以从获得性听力损失中自然恢复，这使它们成为内耳再生研究的理想模型。在这里，我们提供了一个经过验证的禽类耳蜗单细胞 RNA 测序资源。我们描述了三种不同类型感觉毛细胞的特异性标记物，包括一个以前未知的亚群，我们称之为上高毛细胞。我们确定了与高毛细胞相关的支持细胞的标记物，这些细胞代表了禽类内耳的多功能干细胞。同样，我们还提供了位于短耳蜗毛细胞下方的支持细胞的标记物。我们进一步推断了毛细胞基因在耳蜗音高轴上的空间表达梯度。该资源通过为非再生哺乳动物耳蜗的比较研究以及再生禽类耳蜗的纵向研究提供基础，推动了神经生物学、比较生物学和再生医学的发展。
