Abd El-Gawad Ahmed M, El-Amier Yasser A, Bonanomi Giuliano
Botany Department, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt.
Plant Production Department, College of Food & Agriculture Sciences, King Saud University, Saudi Arabia.
Chem Biodivers. 2018 Dec;15(12):e1800392. doi: 10.1002/cbdv.201800392. Epub 2018 Nov 27.
People rely on the synthetic chemical pesticides in most of the agricultural practices, which have a serious problem on human health and ecosystem. Essential oil (EO), derived from wild plants, offers a new opportunity to explore eco-friendly green biocides. This study aimed to characterize the chemical constituents of EO from Egyptian ecospecies of Cleome droserifolia (Forssk.) Delile and evaluate its antioxidant and allelopathic potential. The EO was extracted from aerial parts by hydrodistillation and analyzed by GC/MS. To assess the allelopathic potential, EO concentrations from 50 to 200 μL L were tested on Trifolium repens and three weeds. Antioxidant activity was determined using DPPH. Hydrodistillation yielded 0.64 % of dark yellow oil, which comprises 35 compounds, in which sesquiterpene was a major class and represented by 61.97 % of the total essential oil. Moreover, the dominant sesquiterpenes are cis-nerolidol, α-cadinol, δ-cadinene, and γ-muurolene. The speed of germination index, shoot and root length of clover and weeds were reduced in a concentration-dependent manner. The IC values of C. droserifolia EO on germination of T. repens, Cuscuta trifolii, Melilotus indicus, and Chenopodium murale were 181.6, 183.5, 159.0, and 157.5 μL L , respectively. From the obtained data, we concluded that C. droserifolia EO could provide a hope to produce environment-friendly bioherbicide as well as a natural resource of antioxidants.
在大多数农业生产实践中，人们依赖合成化学农药，这对人类健康和生态系统存在严重问题。从野生植物中提取的精油（EO）为探索环保型绿色生物杀虫剂提供了新机会。本研究旨在表征埃及生态物种白花蛇床子（Cleome droserifolia (Forssk.) Delile）精油的化学成分，并评估其抗氧化和化感潜力。通过水蒸馏法从地上部分提取精油，并采用气相色谱/质谱联用仪进行分析。为评估化感潜力，在白三叶草和三种杂草上测试了浓度为50至200 μL/L的精油。使用二苯基苦味酰基自由基（DPPH）测定抗氧化活性。水蒸馏得到0.64%的深黄色油，其包含35种化合物，其中倍半萜是主要类别，占总精油的61.97%。此外，主要的倍半萜是顺式橙花叔醇、α-杜松醇、δ-杜松烯和γ-依兰油烯。三叶草和杂草的发芽指数、地上部分和根的长度的生长速度呈浓度依赖性降低。白花蛇床子精油对白三叶草、三叶草菟丝子、印度草木犀和墙生藜发芽的半数抑制浓度（IC）值分别为181.6、183.5、159.0和157.5 μL/L。从所得数据中，我们得出结论，白花蛇床子精油有望成为一种环境友好型生物除草剂以及抗氧化剂的天然来源。
