Nabi Masarat, Tabassum Nahida, Ganai Bashir Ahmad
Department of Environmental Science, University of Kashmir, Srinagar, Jammu and Kashmir, India.
Department of Pharmaceutical Sciences, University of Kashmir, Srinagar, Jammu and Kashmir, India.
Front Plant Sci. 2022 Aug 12;13:937946. doi: 10.3389/fpls.2022.937946. eCollection 2022.
The present study aimed to explore the antibacterial activity of various organic root extracts of N.P. Taylor and Airy Shaw and the identification of major functional groups and phytoconstituents through fourier transform infrared spectrometer (FTIR) and gas chromatography-mass spectrometer (GC-MS). The extracts were evaluated for antibacterial activity against multidrug-resistant (MDR) strains ., (MTCC424), (MTCC739), (MTCC139), (MTCC3224), and (MTCC96). ESKAPE pathogens such as , , and are responsible for a majority of all healthcare acquired infections. The ethyl acetate extract showed the highest zone of inhibition against (18 mm) followed by (17 mm). The minimum inhibitory concentration (MIC) of ethyl acetate extract against strain of (4 mg mL) demonstrated therapeutically significant antibacterial activity. The FTIR spectra of root extracts revealed the occurrence of functional characteristic peaks of alcohols, carboxylic acids, aromatic compounds, alkanes, alkenes, and amines that indicates the presence of various metabolites in the extracts. The GC-MS investigation led to the identification of diverse phytoconstituents in each of the extracts with varying concentrations and molecular masses. The highest number of compounds were identified from the methanol extract (112), followed by -hexane extract (88) and ethyl acetate extract (74). The most predominant compounds were 5, 10-pentadecadien-1-ol, ()-(33.94%), -hexadecanoic acid (13.41%) in -hexane extract, 5,10-pentadecadien-1-ol, ()-(10.48%), 1-hexyl-2-nitrocyclohexane (7.94%) in ethyl acetate extract, and 1-hexyl-2-nitrocyclohexane (15.43%), 7,10,13-hexadecatrienal (13.29%) in methanol extract. The results of the present study will create a way for the invention of plant-based medicines for various life-threatening microbial infections using , which may lead to the development of novel drugs against drug-resistant microbial infections.
本研究旨在探索N.P. 泰勒和艾瑞·肖的各种有机根提取物的抗菌活性，并通过傅里叶变换红外光谱仪（FTIR）和气相色谱 - 质谱联用仪（GC - MS）鉴定主要官能团和植物成分。评估了这些提取物对多重耐药（MDR）菌株的抗菌活性，包括金黄色葡萄球菌（MTCC424）、大肠杆菌（MTCC739）、铜绿假单胞菌（MTCC139）、肺炎克雷伯菌（MTCC3224）和鲍曼不动杆菌（MTCC96）。像屎肠球菌、金黄色葡萄球菌和肺炎克雷伯菌这样的ESKAPE病原体是大多数医疗保健相关感染的病因。乙酸乙酯提取物对金黄色葡萄球菌显示出最高的抑菌圈（18毫米），其次是大肠杆菌（17毫米）。乙酸乙酯提取物对金黄色葡萄球菌菌株的最低抑菌浓度（MIC）为4毫克/毫升，显示出具有治疗意义的抗菌活性。根提取物的FTIR光谱揭示了醇、羧酸、芳香化合物、烷烃、烯烃和胺的官能特征峰的出现，这表明提取物中存在各种代谢物。GC - MS研究导致鉴定出每种提取物中具有不同浓度和分子量的多种植物成分。从甲醇提取物中鉴定出的化合物数量最多（112种），其次是正己烷提取物（88种）和乙酸乙酯提取物（74种）。正己烷提取物中最主要的化合物是5,10 - 十五碳二烯 - 1 - 醇，（Z） - （33.94%）、十六烷酸（13.41%）；乙酸乙酯提取物中是5,10 - 十五碳二烯 - 1 - 醇，（Z） - （10.48%）、1 - 己基 - 2 - 硝基环己烷（7.94%）；甲醇提取物中是1 - 己基 - 2 - 硝基环己烷（15.43%）、7,10,13 - 十六碳三烯醛（13.29%）。本研究结果将为利用N.P. 泰勒和艾瑞·肖发明针对各种危及生命的微生物感染的植物性药物创造一条途径，这可能会导致开发出针对耐药性微生物感染的新型药物。
