Lin Zhaoyan, Ku Seockmo, Lim Taehwan, Park Sun Young, Park Myeong Soo, Ji Geun Eog, O'Brien Keely, Hwang Keum Taek
Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University, Seoul 08826, Korea.
Fermentation Science Program, School of Agriculture, College of Basic and Applied Sciences, Middle Tennessee State University, Murfreesboro, TN 37132, USA.
Microorganisms. 2021 Mar 13;9(3):595. doi: 10.3390/microorganisms9030595.
BGN4-SK (BGN4-SK), a recombinant strain which was constructed from BGN4 (BGN4) to produce superoxide dismutase (SOD) and catalase, was analyzed to determine its antioxidant and anti-inflammatory properties in vitro. Culture conditions were determined to maximize the SOD and catalase activities of BGN4-SK. The viability, intracellular radical oxygen species (ROS) levels, intracellular antioxidant enzyme activities, and pro-inflammatory cytokine levels were determined to evaluate the antioxidant and anti-inflammatory activities of BGN4-SK in human intestinal epithelial cells (HT-29) and murine macrophage cells (RAW 264.7). Antioxidant enzymes (SOD and catalase) were produced at the highest levels when BGN4-SK was cultured for 24 h in a medium containing 500 μM MnSO and 30 μM hematin, with glucose as the carbon source. The viability and intracellular antioxidant enzyme activities of HO-stimulated HT-29 treated with BGN4-SK were significantly higher ( < 0.05) than those of cells treated with BGN4. The intracellular ROS levels of HO-stimulated HT-29 cells treated with BGN4-SK were significantly lower ( < 0.05) than those of cells treated with BGN4. BGN4-SK more significantly suppressed the production of interleukin (IL)-6 ( < 0.05), tumor necrosis factor-α ( < 0.01), and IL-8 ( < 0.05) in lipopolysaccharide (LPS)-stimulated HT-29 and LPS-stimulated RAW 264.7 cells compared to BGN4. These results suggest that BGN4-SK may have enhanced antioxidant activities against oxidative stress in HO-stimulated HT-29 cells and enhanced anti-inflammatory activities in LPS-stimulated HT-29 and RAW 264.7 cells.
BGN4-SK（BGN4-SK）是一种由BGN4构建的重组菌株，用于产生超氧化物歧化酶（SOD）和过氧化氢酶，对其体外抗氧化和抗炎特性进行了分析。确定了培养条件以最大化BGN4-SK的SOD和过氧化氢酶活性。测定了人肠上皮细胞（HT-29）和小鼠巨噬细胞（RAW 264.7）的活力、细胞内活性氧（ROS）水平、细胞内抗氧化酶活性和促炎细胞因子水平，以评估BGN4-SK的抗氧化和抗炎活性。当BGN4-SK在含有500μM硫酸锰和30μM血红素的培养基中以葡萄糖作为碳源培养24小时时，抗氧化酶（SOD和过氧化氢酶）的产量最高。用BGN4-SK处理的HO刺激的HT-29的活力和细胞内抗氧化酶活性显著高于（<0.05）用BGN4处理的细胞。用BGN4-SK处理的HO刺激的HT-29细胞的细胞内ROS水平显著低于（<0.05）用BGN4处理的细胞。与BGN4相比，BGN4-SK更显著地抑制了脂多糖（LPS）刺激的HT-29和LPS刺激的RAW 264.7细胞中白细胞介素（IL）-6（<0.05）、肿瘤坏死因子-α（<0.01）和IL-8（<0.05）的产生。这些结果表明，BGN4-SK可能对HO刺激的HT-29细胞中的氧化应激具有增强的抗氧化活性，并在LPS刺激的HT-29和RAW 264.7细胞中具有增强的抗炎活性。
