帕卢谷中葱（荒木）品种对伤寒杆菌ATCC 27870的抗菌活性及计算机模拟评估

Antibacterial activity of Shallots ( Araki.) cultivars in Palu Valley against Typhi ATCC 27870 through and in silico evaluation.

作者信息

Sari Sitti Rahmah, Wardhani Riuh, Umar Faiqah, Husain Dirayah Rauf, Iwansyah Ade Chandra

机构信息

Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University, South Sulawesi, Indonesia.

Department of Animal and Dairy Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon, Republic of Korea.

出版信息

Iran J Microbiol. 2024 Apr;16(2):208-218. doi: 10.18502/ijm.v16i2.15354.

DOI:10.18502/ijm.v16i2.15354

PMID:38854984

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11162162/

Abstract

BACKGROUND AND OBJECTIVES

Shallots, recognized for their minimal toxicity, cost-effectiveness, and widespread availability, are increasingly considered a viable source of biological activity. This study evaluates the antibacterial efficacy of a specific shallot cultivar from Palu Valley, Indonesia, against , the pathogen responsible for typhoid fever.

MATERIALS AND METHODS

Utilizing thin-layer chromatography (TLC-bioautography) and gas chromatography-mass spectroscopy (GC-MS), the study identifies active compounds in shallot ethanol extract and employs molecular docking to assess interactions between receptors and ligands.

RESULTS

Findings indicate significant antibacterial activity, with a notable inhibition zone diameter of 31.5 mm at spot Rf 0.28 in TLC bioautography and an optimum concentration of 2% yielding an average clear zone diameter of 28.27 mm in the agar diffusion test. GC-MS analysis reveals 41 compounds, predominantly dodecanoic acid and 1,2,3-propanetriyl ester. Additionally, molecular docking reveals the lowest binding affinity (-7.3 kcal/mol) for Ergost-8-En-3-Ol, 14-Methyl-, (3.Beta,5. Alpha.) against DNA gyrase.

CONCLUSION

This study confirms Palu Valley shallot extract's potent antibacterial effect against highlighting its therapeutic potential.

摘要

背景与目的

青葱因其毒性极小、成本效益高且广泛可得，越来越被视为一种具有生物活性的可行来源。本研究评估了印度尼西亚帕卢谷的一种特定青葱品种对导致伤寒热的病原体的抗菌效果。

材料与方法

该研究利用薄层色谱法（TLC - 生物自显影）和气相色谱 - 质谱联用（GC - MS）来鉴定青葱乙醇提取物中的活性化合物，并采用分子对接来评估受体与配体之间的相互作用。

结果

研究结果表明具有显著的抗菌活性，在TLC生物自显影中，Rf值为0.28处的斑点的抑菌圈直径为31.5毫米，在琼脂扩散试验中，2%的最佳浓度产生的平均透明圈直径为28.27毫米。GC - MS分析揭示了41种化合物，主要是十二烷酸和1,2,3 - 丙三醇酯。此外，分子对接显示Ergost - 8 - En - 3 - Ol，14 - Methyl -，(3.Beta,5.Alpha.)对DNA促旋酶的结合亲和力最低（-7.3千卡/摩尔）。