Rezaei Robabeh, Aliannejad Rasoul, Falahati Mehraban, Ghasemi Zeinab, Ashrafi-Khozani Mahtab, Fattahi Mahsa, Razavi Tandis, Farahyar Shirin
Microbial Biotechnology Research Center (MBiRC), School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Iran J Microbiol. 2024 Apr;16(2):273-279. doi: 10.18502/ijm.v16i2.15362.
The presence of fungi in the respiratory tract as mycobiome, particularly species (spp.), remains a serious problem due to increasing numbers of immunocompromised patients. The confirmed reliable existence of these pathogens due to frequent colonization is essential. This investigation aimed to recognize spp. among isolates from bronchoalveolar lavage of immunocompromised and critically ill patients and to evaluate their susceptibility to antimycotic drugs.
Bronchoalveolar lavage fluid was collected from 161 hospitalized patients presenting with suspected respiratory fungal infection /colonization. The specimens were examined by standard molecular and mycological assays. spp. were recognized with sequence assessment of the D1-D2 section of the large subunit ribosomal DNA. The susceptibility of isolates to common antimycotic drugs was distinguished by standard broth microdilution.
Seventy-one clinical isolates of spp. were recognized. was the most frequent, followed by (), and We found 5.1% of isolates and 8% of isolates to show resistance to fluconazole. The whole of the spp. were sensitive to amphotericin B and caspofungin.
This study demonstrated that and are the most common isolates of bronchoalveolar lavage fluid in patients, and the drug susceptibility screening confirmed that amphotericin B and caspofungin are effective against spp. but some and isolates showed resistance to fluconazole.
由于免疫功能低下患者数量的增加，呼吸道中作为真菌群落的真菌尤其是某些菌种的存在仍然是一个严重问题。由于频繁定植，确认这些病原体的可靠存在至关重要。本研究旨在识别免疫功能低下和重症患者支气管肺泡灌洗分离株中的某些菌种，并评估它们对抗真菌药物的敏感性。
从161例疑似呼吸道真菌感染/定植的住院患者中采集支气管肺泡灌洗液。通过标准分子和真菌学检测对标本进行检查。通过对大亚基核糖体DNA的D1-D2区进行序列评估来识别某些菌种。通过标准肉汤微量稀释法区分分离株对常见抗真菌药物的敏感性。
识别出71株某些菌种的临床分离株。某菌最为常见，其次是另一菌（括号内为菌种名）、又一菌和再一菌。我们发现5.1%的某菌分离株和8%的另一菌分离株对氟康唑耐药。所有的某些菌种对两性霉素B和卡泊芬净敏感。
本研究表明，某菌和另一菌是患者支气管肺泡灌洗液中最常见的分离株，药敏筛查证实两性霉素B和卡泊芬净对某些菌种有效，但一些某菌和另一菌分离株对氟康唑耐药。
