超分辨率表面增强拉曼光谱生物成像
Super-Resolution SERS Spectral Bioimaging.
作者信息
Schultz Zachary D, Shoup Deben N, Smith Abigail E
机构信息
Department of Chemistry and Biochemistry, The Ohio State University, 100 W. 18 Avenue, Columbus, OH, USA 43210-1173.
出版信息
Proc SPIE Int Soc Opt Eng. 2022 Aug;12203. doi: 10.1117/12.2632824. Epub 2022 Oct 3.
Advances in nanotechnology enable the detection of trace molecules from the enhanced Raman signal generated at the surface of plasmonic nanoparticles. We have developed technology to enable super-resolution imaging of plasmonic nanoparticles, where the fluctuations in the surface enhanced Raman scattering (SERS) signal can be analyzed with localization microscopy techniques to provide nanometer spatial resolution of the emitting molecule's location. Additional work now enables the super-resolved SERS image and the corresponding spectrum to be acquired simultaneously. Here we will discuss how this approach can be applied to provide new insights into biological cells.
纳米技术的进步使得能够从等离子体纳米颗粒表面产生的增强拉曼信号中检测痕量分子。我们已经开发出技术来实现等离子体纳米颗粒的超分辨率成像，其中表面增强拉曼散射（SERS）信号的波动可以通过定位显微镜技术进行分析，以提供发射分子位置的纳米级空间分辨率。现在的进一步工作使得能够同时获取超分辨SERS图像和相应的光谱。在这里，我们将讨论这种方法如何应用于为生物细胞提供新的见解。
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