Zhang Chuyue, Wang Chongwen, Xiao Rui, Tang Li, Huang Jing, Wu Di, Liu Shuwen, Wang Yong, Zhang Dong, Wang Shengqi, Chen Xiangmei
Department of Nephrology, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Center for Kidney Diseases, Beijing Key Laboratory of Kidney Diseases, Beijing 100853, China.
J Mater Chem B. 2018 Jun 14;6(22):3751-3761. doi: 10.1039/c8tb00504d. Epub 2018 May 25.
A sensitive surface-enhanced Raman scattering (SERS) platform for the simultaneous detection of two pathogenic bacteria on the basis of dual recognition by vancomycin and aptamers is reported for the first time, using Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) as target bacteria. This dual-recognition platform was developed using vancomycin-modified FeO@Au magnetic nanoparticles (FeO@Au-Van MNPs) for broad-spectrum recognition and efficient bacterial enrichment, and aptamer-functionalized SERS tags for the specific and sensitive quantification of target bacteria in authentic specimens. Our results showed that the FeO@Au-Van MNPs allow the rapid capture of bacteria from complex solutions with a capture efficiency as high as 88.89% for S. aureus and 74.96% for E. coli within 15 min. The limits of detection (LODs) of the platform were found to be 20 and 50 cells mL for S. aureus and E. coli, respectively, and favorable linear relations were observed between the logarithm of bacterial concentration and the SERS intensity at the characteristic Raman peaks. The high specificity and potential clinical applications of the technique were also proven. Our method elucidated novel advances and potential applications in the field of multiple SERS detection of pathogens.
首次报道了一种基于万古霉素和适配体双重识别的灵敏表面增强拉曼散射（SERS）平台，用于同时检测两种病原菌，以大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）作为目标细菌。该双重识别平台利用万古霉素修饰的FeO@Au磁性纳米颗粒（FeO@Au-Van MNPs）进行广谱识别和高效细菌富集，并使用适配体功能化的SERS标签对真实样本中的目标细菌进行特异性和灵敏定量。我们的结果表明，FeO@Au-Van MNPs能够在15分钟内从复杂溶液中快速捕获细菌，对金黄色葡萄球菌的捕获效率高达88.89%，对大肠杆菌的捕获效率为74.96%。该平台对金黄色葡萄球菌和大肠杆菌的检测限分别为20和50个细胞/mL，并且在细菌浓度对数与特征拉曼峰处的SERS强度之间观察到良好的线性关系。该技术的高特异性和潜在临床应用也得到了证实。我们的方法阐明了在病原菌多重SERS检测领域的新进展和潜在应用。
