Department of Clinical Laboratory, Shanghai Yangsi Hospital, Shanghai, 200126, China.
Department of Ultrasound, Central Hospital Affiliated to Shandong First Medical University, Jinan, 250013, China.
Tuberculosis (Edinb). 2024 Sep;148:102530. doi: 10.1016/j.tube.2024.102530. Epub 2024 Jun 6.
To determine the usefulness of LINC00152 and LARS2-AS1 as potential biomarkers for latent tuberculosis (LTB) and active tuberculosis (ATB), as well as their effect on Mycobacterium (Mtb) infection.
The expression levels of LINC00152 and LARS2-AS1 in the health, patients with LTB and ATB were detected by qRT-PCR. The ROC curves were constructed to show their potential as biomarkers. The intracellular survival assays for Mtb and the levels of immune-related cytokines were determined to discover the effect of LINC00152 and LARS2-AS1 on Mtb infection. The relationships of miR-485-5p with LINC00152 and LARS2-AS1 were explored.
LINC00152 and LARS2-AS1 levels were significantly elevated in patients with ATB and LTB, and Mtb-infected macrophages. LINC00152 and LARS2-AS1 can distinguish the LTB from the health and ATB from LTB. LARS2-AS1 and LINC00152 knock-down reduced the intracellular Mtb survival and induced cellular immune response after Mtb challenge. miR-485-5p was a targeting miRNA for LINC00152 and LARS2-AS1.
LINC00152 and LARS2-AS1 can be considered as potential biomarkers for tuberculosis disease. LINC00152 and LARS2-AS1 have anti-Mtb effects.
确定 LINC00152 和 LARS2-AS1 作为潜伏性结核病 (LTB) 和活动性结核病 (ATB) 潜在生物标志物的有用性，以及它们对分枝杆菌 (Mtb) 感染的影响。
通过 qRT-PCR 检测健康、LTB 和 ATB 患者中 LINC00152 和 LARS2-AS1 的表达水平。构建 ROC 曲线以显示其作为生物标志物的潜力。进行 Mtb 细胞内存活测定和免疫相关细胞因子水平测定，以发现 LINC00152 和 LARS2-AS1 对 Mtb 感染的影响。探讨 miR-485-5p 与 LINC00152 和 LARS2-AS1 的关系。
ATB 和 LTB 患者以及 Mtb 感染的巨噬细胞中 LINC00152 和 LARS2-AS1 水平显著升高。LINC00152 和 LARS2-AS1 可区分 LTB 与健康人群和 ATB 与 LTB。LARS2-AS1 和 LINC00152 敲低可减少细胞内 Mtb 存活并在 Mtb 攻击后诱导细胞免疫反应。miR-485-5p 是 LINC00152 和 LARS2-AS1 的靶向 miRNA。
LINC00152 和 LARS2-AS1 可被视为结核病的潜在生物标志物。LINC00152 和 LARS2-AS1 具有抗 Mtb 作用。
