Tozaki Nagie, Tawada Chisato, Tanaka Kayoko, Im Dongjun, Ueda Keisuke, Kato Noriko, Tsuji Hiromu, Yoshie Yuka, Matsuo Maho, Ichiki Naohisa, Niwa Hirofumi, Mizutani Yoko, Shu En, Iwata Hiroaki
Department of Dermatology, Gifu University Graduate School of Medicine, Gifu, Japan.
JID Innov. 2024 Apr 16;4(4):100282. doi: 10.1016/j.xjidi.2024.100282. eCollection 2024 Jul.
ROS are involved in the pathogenesis of bullous pemphigoid (BP), but this involvement has not been fully elucidated. In this study, to further elucidate the pathogenic role of ROS in BP, we examined the results of the diacron-reactive oxygen metabolite test and the biological antioxidant potential test for 16 patients with BP who visited our hospital before being treated with systemic corticosteroids. In the patients with BP, the average diacron-reactive oxygen metabolite levels, expressed in Carratelli units, were significantly reduced at 1 month of treatment (from 335.6 ± 40.3 Carratelli units to 224.7 ± 61.6 Carratelli units, < .001). Bullous Pemphigoid Disease Area Index (erosions/blisters) scores correlated with diacron-reactive oxygen metabolite levels ( = 0.51), suggesting that those levels reflect the disease severity. We also performed staining of 3,5-dibromotyrosine in skin tissues. The 3,5-dibromotyrosine is expected to be a marker of tissue damage related to inflammation and allergies. The 3,5-dibromotyrosine was stained in infiltrated cells around the dermis, throughout the blister fluid, and at the basement membrane within the blister. It is considered that tissue destruction caused by the myeloperoxidase released from neutrophils and by eosinophil peroxidase released from eosinophils is involved in blister formation. The results suggest that ROS play a role in BP.
活性氧（ROS）参与大疱性类天疱疮（BP）的发病机制，但这种参与尚未完全阐明。在本研究中，为了进一步阐明ROS在BP中的致病作用，我们检查了16例在接受全身皮质类固醇治疗前来我院就诊的BP患者的戴克隆活性氧代谢物测试结果和生物抗氧化潜力测试结果。在BP患者中，以卡拉泰利单位表示的平均戴克隆活性氧代谢物水平在治疗1个月时显著降低（从335.6±40.3卡拉泰利单位降至224.7±61.6卡拉泰利单位，P<0.001）。大疱性类天疱疮疾病面积指数（糜烂/水疱）评分与戴克隆活性氧代谢物水平相关（r=0.51），表明这些水平反映了疾病的严重程度。我们还对皮肤组织中的3,5-二溴酪氨酸进行了染色。3,5-二溴酪氨酸有望成为与炎症和过敏相关的组织损伤的标志物。在真皮周围的浸润细胞、整个水疱液以及水疱内的基底膜处均检测到3,5-二溴酪氨酸染色。据认为，中性粒细胞释放的髓过氧化物酶和嗜酸性粒细胞释放的嗜酸性粒细胞过氧化物酶引起的组织破坏参与了水疱的形成。结果表明ROS在BP中起作用。
