Weber M, Köhler H, Manns M, Meyer zum Büschenfelde K H
Clin Immunol Immunopathol. 1985 Jun;35(3):285-94. doi: 10.1016/0090-1229(85)90090-x.
An enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to human glomerular basement membrane has been developed. Special emphasis has been put on the choice of microtiter plates which were coated with a collagenase digest of human glomerular basement membrane. Results differed markedly between the different microtiter plates. Best results were obtained with a flexible polyvinylchloride microtiter plate with flat wells (Dynatec). This plate exhibited the highest positive/negative ratio and the lowest intraassay standard deviation. Optimal conditions for each step in the ELISA have been determined. The assay proved to be specific, sensitive, and reproducible. Circulating antibodies in each of 11 patients with active antiglomerular basement membrane disease were detected by the ELISA, while sera from patients with various renal and nonrenal diseases were negative by the test.
已开发出一种用于检测抗人肾小球基底膜抗体的酶联免疫吸附测定（ELISA）。特别强调了对包被有人肾小球基底膜胶原酶消化物的微量滴定板的选择。不同的微量滴定板之间结果差异显著。使用带有平底孔的柔性聚氯乙烯微量滴定板（Dynatec）可获得最佳结果。该板显示出最高的阳性/阴性比和最低的批内标准差。已确定ELISA中每个步骤的最佳条件。该测定方法具有特异性、敏感性和可重复性。通过ELISA检测了11例活动性抗肾小球基底膜病患者中每例患者的循环抗体，而各种肾脏和非肾脏疾病患者的血清检测结果均为阴性。
