一种使用微波测量抗肾小球基底膜抗体的快速酶联免疫吸附测定法。
A rapid ELISA for measurement of anti-glomerular basement membrane antibodies using microwaves.
作者信息
van Dorp R, Daha M R, Muizert Y, Marani E, Boon M E
机构信息
Leiden Cytology and Pathology Laboratory, The Netherlands.
出版信息
J Clin Lab Immunol. 1993;40(3):135-47.
The presence of anti-glomerular basement membrane antibodies is one of the features of Goodpasture's syndrome. Since the disease has a rapidly progressive course, an early diagnosis is essential. As was already demonstrated in other ELISA methods, 2.45-GHz microwave irradiation can accelerate all kinds of time consuming processes in several laboratory techniques. The application of microwaves in an ELISA for the measurement of anti-GBM antibodies in serum indicated that a considerable time reduction of 75% can be achieved, resulting in a rapid and reliable assay. In addition, microwaves can also have a positive effect on the resolution of that particular ELISA as shown in this study.
抗肾小球基底膜抗体的存在是古德帕斯彻综合征的特征之一。由于该疾病病程进展迅速，早期诊断至关重要。正如其他酶联免疫吸附测定（ELISA）方法所证实的那样，2.45吉赫兹微波辐射可以加速多种实验室技术中耗时的各种过程。在用于测量血清中抗肾小球基底膜抗体的ELISA中应用微波表明，可以将时间大幅减少75%，从而实现快速且可靠的检测。此外，如本研究所示，微波对该特定ELISA的分辨率也可产生积极影响。
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