Fish A J, Kleppel M, Jeraj K, Michael A F
J Lab Clin Med. 1985 Jun;105(6):700-5.
An immunoassay has been developed to detect anti-glomerular basement membrane (GBM) antibodies in human sera. Various plating conditions, types of microtiter plates, and the use of biotinylated or peroxidase-labeled secondary antibodies were examined. The described assay is reliable, fast, and convenient. Sera with positive reactivity in anti-GBM nephritis and Goodpasture's syndrome are readily discriminated from sera obtained from normal individuals or patients with a variety of other diseases.
已开发出一种免疫测定法来检测人血清中的抗肾小球基底膜（GBM）抗体。研究了各种包被条件、微量滴定板类型以及生物素化或过氧化物酶标记二抗的使用情况。所描述的测定法可靠、快速且方便。抗GBM肾炎和肺出血肾炎综合征中具有阳性反应性的血清很容易与正常个体或患有各种其他疾病的患者的血清区分开来。
