Tang Yantian, Chen Si, Chen Lingli, Ouyang Kehui, Chen Hui, Wang Wenjun
College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, China.
School of Life Science and Pharmacy, Jiujiang University, Jiujiang, China.
Front Vet Sci. 2024 May 27;11:1381188. doi: 10.3389/fvets.2024.1381188. eCollection 2024.
In this study, the polysaccharides (PCPs) were heteropolysaccharides with molecular weights of 63.17 kDa and 8.99 kDa, and their total carbohydrate content was 76.17 ± 0.23%, uronic acid content was 19.92 ± 0.42%, and protein content was 1.24 ± 0.07%. PCP is composed of arabinose, galactose, glucose, and glucuronic acid, with a molar ratio of 0.196:0.249:0.451:0.104. In addition, we further investigated the effects of the diet supplemented with different doses of PCP on growth performance, meat quality, and anti-oxidant capacity in Chongren Partridge chickens. A total of 200 chickens were randomly allocated into 4 treatments, and fed with a basal diet of 0 (CON), 200 (LPCP), 400 (MPCP), and 800 (HPCP) mg/kg PCP for a 14-day prefeeding period and a formal experimental period of 56 days. Results showed that dietary PCP significantly increased final body weight (BW), average daily gain (ADG), and decreased feed-to-gain ratio (F/G) from days 1 to 56. Meanwhile, dietary PCP reduced yellowness (b) values and increased redness (a) values at 24 h in breast muscles ( < 0.05). Furthermore, LPCP and MPCP significantly increased the level of guanylic acid (GMP) ( < 0.05). MPCP increased the content of free amino acids (isoleucine, leucine, lysine, methionine, threonine, valine, alanine, glutamic acid, serine, cysteine), total essential amino acid (EAA), total flavor amino acid (FAA), total AA, the content of fatty acids (c14:1, c16:1, and c22:2), and monounsaturated fatty acids (MUFAs) in the breast muscle when compared to CON ( < 0.05). In addition, MPCP significantly reduced the content of malondialdehyde (MDA) and increased the transcript abundances of fatty acid desaturase 2 (), fatty acid synthase (), lipoprotein lipase (), and sterol regulatory element binding protein-1 () in the breast muscles of the chickens ( < 0.05). In light of the aforementioned results, PCP at 400 mg/kg could be used as an effective additive because it not only promotes the growth performance of Chongren Partridge chickens but also shows a conducive role in meat quality, especially in meat flavor.
在本研究中，多糖（PCPs）为杂多糖，分子量分别为63.17 kDa和8.99 kDa，其总碳水化合物含量为76.17±0.23%，糖醛酸含量为19.92±0.42%，蛋白质含量为1.24±0.07%。PCP由阿拉伯糖、半乳糖、葡萄糖和葡萄糖醛酸组成，摩尔比为0.196:0.249:0.451:0.104。此外，我们进一步研究了添加不同剂量PCP的日粮对崇仁鹧鸪生长性能、肉质和抗氧化能力的影响。总共200只鸡被随机分为4组，在14天的预饲期和56天的正式试验期内，分别饲喂含0（CON）、200（LPCP）、400（MPCP）和800（HPCP）mg/kg PCP的基础日粮。结果表明，日粮PCP显著提高了第1至56天的末体重（BW）、平均日增重（ADG），并降低了料重比（F/G）。同时，日粮PCP降低了胸肌24小时时的黄度（b）值，提高了红度（a）值（P<0.05）。此外，LPCP和MPCP显著提高了鸟苷酸（GMP）水平（P<0.05）。与CON组相比，MPCP提高了胸肌中游离氨基酸（异亮氨酸、亮氨酸、赖氨酸、蛋氨酸、苏氨酸、缬氨酸、丙氨酸、谷氨酸、丝氨酸、半胱氨酸）、总必需氨基酸（EAA）、总风味氨基酸（FAA）、总氨基酸、脂肪酸（c14:1、c16:1和c22:2）以及单不饱和脂肪酸（MUFA）的含量（P<0.05）。此外，MPCP显著降低了鸡胸肌中丙二醛（MDA）的含量，并提高了脂肪酸去饱和酶2（FADS2）、脂肪酸合酶（FASN）、脂蛋白脂肪酶（LPL）和固醇调节元件结合蛋白-1（SREBP-1）的转录丰度（P<0.05）。根据上述结果，400 mg/kg的PCP可作为一种有效的添加剂，因为它不仅能促进崇仁鹧鸪的生长性能，而且对肉质，尤其是肉香味有促进作用。
