Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
Int J Biol Macromol. 2020 Jul 15;155:61-70. doi: 10.1016/j.ijbiomac.2020.03.198. Epub 2020 Mar 26.
The present study was aimed to investigate the effects of the addition of Yingshan Yunwu green tea polysaccharide conjugates (GTPC) on meat quality, immune response and gut microflora in chickens. A total of 200 chickens with average initial body weight were randomly allotted to 4 groups. Intestinal samples were collected at the end of experiment for bacterial culture and microbial community analysis by 16S rDNA gene sequencing using Illumina MiSeq. Chicken breast muscle and serum were also sampled for analysis of meat quality and immune function. The results showed that dietary GTPC addition increased (P < 0.05) chicken breast muscle pH and redness-greenness (a*) value and decreased (P < 0.05) the values of lightness (L*), yellowness-blueness (b*), hardness, toughness and adhesiveness. In addition, dietary supplementation of GTPC increased (P < 0.05) the weight of thymus and bursa and serum concentrations of IgA and IgG. Furthermore, of the 10 bacterial phyla, the predominant taxa across all sampling time-points were Bacteroidetes, Firmicutes, Proteobacteria, and Deferribacteres, representing >97% of all sequences. GTPC increased the abundance of Bacteroidetes and Lactobacillus, and decreased the abundance of Proteobacteria. These findings provided some references of the application of GTPC in the poultry industry.
本研究旨在探讨英山云雾绿茶多糖缀合物（GTPC）的添加对鸡的肉质、免疫反应和肠道微生物群的影响。将 200 只平均初始体重的鸡随机分为 4 组。实验结束时，通过 Illumina MiSeq 对肠道样本进行细菌培养和 16S rDNA 基因测序进行微生物群落分析。还采集鸡胸肉和血清进行肉质和免疫功能分析。结果表明，日粮 GTPC 添加增加了（P < 0.05）鸡胸肉的 pH 值和红绿色（a*）值，降低了（P < 0.05）亮度（L*）、黄蓝色（b*）、硬度、韧性和粘性值。此外，日粮补充 GTPC 增加了（P < 0.05）胸腺和法氏囊的重量以及血清 IgA 和 IgG 的浓度。此外，在所有采样时间点，10 个细菌门中占主导地位的分类群是拟杆菌门、厚壁菌门、变形菌门和脱硫菌门，代表所有序列的>97%。GTPC 增加了拟杆菌门和乳酸菌的丰度，降低了变形菌门的丰度。这些发现为 GTPC 在家禽业中的应用提供了一些参考。
