脐带血中的微小RNA与足月儿发育：一项前瞻性研究。

MicroRNAs in Umbilical Cord Blood and Development in Full-Term Newborns: A Prospective Study.

作者信息

Wang Liang-Jen, Tsai Ching-Chang, Chao How-Ran, Lee Sheng-Yu, Chen Chih-Cheng, Li Sung-Chou

机构信息

Department of Child and Adolescent Psychiatry, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.

Institute for Translational Research in Biomedicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan.

出版信息

Biomark Insights. 2024 Jun 10;19:11772719241258017. doi: 10.1177/11772719241258017. eCollection 2024.

DOI:10.1177/11772719241258017

PMID:38863527

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11165956/

Abstract

BACKGROUND

Exploring the epigenetic regulations, such as microRNA, in newborns holds significant promise for enhancing our ability to address and potentially prevent early-life developmental delays.

OBJECTIVES

Hence, this research seeks to investigate if the expression of miRNA in the umbilical cord blood of infants can forecast their developmental outcomes as they grow older.

DESIGN AND METHOD

We enrolled 143 full-term newborns, delivered either via cesarean section (CS) or through natural spontaneous delivery (NSD). We then analyzed the profiles of specific miRNAs (miR-486-5p, miR-126-5p, miR-140-3p, miR-151a-3p, miR-142-5p, and miR-30e-5p) in the umbilical cord blood of these infants. Subsequently, we performed follow-up assessments using Bayley-III scores when the cohort reached 1 year of age. Furthermore, we conducted pathway-enrichment analyses on the target genes associated with these examined miRNAs.

RESULTS

When comparing newborns delivered via cesarean section (CS) to those born via natural spontaneous delivery (NSD), we observed notable differences. Specifically, newborns through NSD displayed significantly higher ΔCt values for miR-486-5p, alongside lower ΔCt values for miR-126-5p and miR-151a-3p in their cord blood. At 1 year of age, cognitive development was significantly linked to the ΔCt values of miR-140-3p and miR-142-5p, while language development showed a significant association with the ΔCt values of miR-140-3p. Moreover, our pathway enrichment analyses revealed that the target genes of these miRNAs were consistently involved in the pathways related to neurons, such as axon guidance and the neurotrophin signaling pathway.

CONCLUSION

In summary, this study represents a pioneering effort in elucidating the potential connections between miRNA levels in cord blood and the health indicators and neurodevelopment of newborns at 1 year of age. Our findings underscore the significance of miRNA levels at birth in influencing mechanisms related to neurodevelopment.

摘要

背景

探索新生儿中的表观遗传调控，如微小RNA，对于提高我们应对并潜在预防早期发育迟缓的能力具有重大前景。

目的

因此，本研究旨在调查婴儿脐带血中微小RNA的表达是否能够预测其长大后的发育结果。

设计与方法

我们招募了143名足月新生儿，他们通过剖宫产（CS）或自然顺产（NSD）分娩。然后我们分析了这些婴儿脐带血中特定微小RNA（miR-486-5p、miR-126-5p、miR-140-3p、miR-151a-3p、miR-142-5p和miR-30e-5p）的谱。随后，当该队列达到1岁时，我们使用贝利婴幼儿发展量表第三版（Bayley-III）评分进行随访评估。此外，我们对与这些检测的微小RNA相关的靶基因进行了通路富集分析。

结果

将剖宫产（CS）分娩的新生儿与自然顺产（NSD）出生的新生儿进行比较时，我们观察到显著差异。具体而言，通过自然顺产的新生儿脐带血中miR-486-5p的ΔCt值显著更高，而miR-126-5p和miR-151a-3p的ΔCt值更低。在1岁时，认知发育与miR-140-3p和miR-142-5p的ΔCt值显著相关，而语言发育与miR-140-3p的ΔCt值显著相关。此外，我们的通路富集分析表明，这些微小RNA的靶基因始终参与与神经元相关的通路，如轴突导向和神经营养因子信号通路。