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探索……中番茄黄化曲叶病毒抗性的来源。 你提供的原文似乎不完整，句末的“in.”后面应该还有具体内容。

Exploring the source of TYLCV resistance in .

作者信息

Hayashi Satomi, Souvan Jacqueline M, Bally Julia, de Felippes Felipe F, Waterhouse Peter M

机构信息

Centre for Agriculture and the Bioeconomy, Queensland University of Technology, Brisbane, QLD, Australia.

Australian Research Council Centre of Excellence for Plant Success in Nature and Agriculture, Queensland University of Technology, Brisbane, QLD, Australia.

出版信息

Front Plant Sci. 2024 May 28;15:1404160. doi: 10.3389/fpls.2024.1404160. eCollection 2024.

DOI:10.3389/fpls.2024.1404160
PMID:38863537
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11165019/
Abstract

Tomato Yellow Leaf Curl Virus (TYLCV) is one of the most devastating pathogens of tomato, worldwide. It is vectored by the globally prevalent whitefly, Bemisia tabaci, and is asymptomatic in a wide range of plant species that act as a virus reservoir. The most successful crop protection for tomato in the field has been from resistance genes, of which five loci have been introgressed fromwild relatives. Of these, the Ty-1/Ty-3 locus, which encodes an RNA-dependent RNA polymerase 3 (RDR3), has been the most effective. Nevertheless, several TYLCV strains that break this resistance are beginning to emerge, increasing the need for new sources of resistance. Here we use segregation analysis and CRISPR-mediated gene dysfunctionalisation to dissect the differential response of two isolates of Nicotiana benthamiana to TYLCV infection. Our study indicates the presence of a novel non-RDR3, but yet to be identified, TYLCV resistance gene in a wild accession of . This gene has the potential to be incorporated into tomatoes.

摘要

番茄黄化曲叶病毒（TYLCV）是全球范围内番茄最具毁灭性的病原体之一。它由全球普遍存在的烟粉虱传播，并且在作为病毒宿主的多种植物物种中无症状。田间对番茄最成功的作物保护措施来自抗性基因，其中五个位点已从野生近缘种导入。其中，编码RNA依赖性RNA聚合酶3（RDR3）的Ty-1/Ty-3位点最为有效。然而，一些能打破这种抗性的TYLCV菌株开始出现，增加了对新抗性来源的需求。在这里，我们使用分离分析和CRISPR介导的基因功能缺失来剖析本氏烟草两个分离株对TYLCV感染的差异反应。我们的研究表明，在一个野生烟草种质中存在一个新的、尚未鉴定的非RDR3 TYLCV抗性基因。该基因有潜力被整合到番茄中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/11165019/d79f4341b273/fpls-15-1404160-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/11165019/7b7c9e6e6fd1/fpls-15-1404160-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/11165019/0decbb1885dd/fpls-15-1404160-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/11165019/8b57b9f579bd/fpls-15-1404160-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/11165019/ab1b4fe7bbec/fpls-15-1404160-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/11165019/e558b1caed28/fpls-15-1404160-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/11165019/acfa6d19b3b9/fpls-15-1404160-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/11165019/d79f4341b273/fpls-15-1404160-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/11165019/7b7c9e6e6fd1/fpls-15-1404160-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/11165019/0decbb1885dd/fpls-15-1404160-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/11165019/8b57b9f579bd/fpls-15-1404160-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/11165019/ab1b4fe7bbec/fpls-15-1404160-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/11165019/e558b1caed28/fpls-15-1404160-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/11165019/acfa6d19b3b9/fpls-15-1404160-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d96/11165019/d79f4341b273/fpls-15-1404160-g007.jpg

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Nat Rev Mol Cell Biol. 2022 Oct;23(10):645-662. doi: 10.1038/s41580-022-00496-5. Epub 2022 Jun 16.
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PepYLCIV and PepYLCAV resistance gene Pepy-2 encodes DFDGD-Class RNA-dependent RNA polymerase in Capsicum.辣椒中的 PepYLCIV 和 PepYLCAV 抗性基因 Pepy-2 编码 DFDGD 类 RNA 依赖的 RNA 聚合酶。
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