Confers Broad-Spectrum Resistance to Geminiviruses.

The selection of resistant crops is an effective method for controlling geminivirus diseases. encodes a messenger RNA surveillance factor Pelota with a single amino acid mutation (Pelota), which confers effective resistance to tomato yellow leaf curl virus (TYLCV). No studies have investigated whether confers resistance to other geminiviruses. Here, we demonstrate that the tomato line exhibits effective resistance to various geminiviruses. It confers resistance to two representative begomoviruses, tomato yellow leaf curl China virus/tomato yellow leaf curl China betasatellite complex and tomato leaf curl Yunnan virus. The line also exhibits partial resistance to a curtovirus beet curly top virus. Importantly, confers resistance to TYLCV with a betasatellite. Southern blotting and quantitative polymerase chain reaction analyses showed that significantly less DNA of these geminiviruses accumulated in the line than in the susceptible line. Moreover, knockdown of expression converted a plant from a geminivirus-susceptible host to a geminivirus-resistant host. Overall, our findings suggest that is an important resistance gene resource for crop breeding to control geminiviruses.