对该基因家族的综合分析揭示了其在植物发育中的调控作用。

Comprehensive analysis of the gene family reveals the regulatory role of in plant development.

作者信息

Yang Maogeng, Chen Shoukun, Geng Jiahui, Gao Shuqiang, Chen Shihua, Li Huihui

机构信息

Key Laboratory of Plant Molecular & Developmental Biology, College of Life Sciences, Yantai University, Yantai, Shandong, China.

State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.

出版信息

Front Plant Sci. 2024 May 28;15:1390461. doi: 10.3389/fpls.2024.1390461. eCollection 2024.

DOI:10.3389/fpls.2024.1390461

PMID:38863548

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11165199/

Abstract

INTRODUCTION

The gene family, prevalent in eukaryotes, assumes diverse roles in cellular processes. , a halophyte with exceptional salt tolerance, flood tolerance, reproduction, and diffusion ability, offers great potential for industrial applications and crop breeding analysis. The exploration of growth and development-related genes in this species offers immense potential for enhancing crop yield and environmental adaptability, particularly in industrialized plantations. However, the understanding of their role in regulating plant growth and development remains limited.

METHODS

In this study, we conducted a comprehensive analysis of genes in at the whole-genome level, delving into their characteristics such as physicochemical properties, phylogenetic relationships, gene architecture, and expression patterns. Additionally, we cloned the gene, a gene in plant growth and development across diverse species.

RESULTS

We identified a total of 582 WD40 proteins in the genome, exhibiting an uneven distribution across chromosomes. Through phylogenetic analysis, we categorized the 582 SaWD40 proteins into 12 distinct clades. Examining the duplication patterns of genes, we observed a predominant role of segmental duplication in their expansion. A substantial proportion of gene duplication pairs underwent purifying selection through evolution. To explore the functional aspects, we selected , a homolog of , for overexpression in Arabidopsis. Subcellular localization analysis revealed that the SaTTG1 protein localized in the nucleus and plasma membrane, exhibiting transcriptional activation in yeast cells. The overexpression of in resulted in early flowering and increased seed size.

DISCUSSION

These outcomes significantly contribute to our understanding of WD40 gene functions in halophyte species. The findings not only serve as a valuable foundation for further investigations into genes in halophyte but also offer insights into the molecular mechanisms governing plant development, offering potential avenues in molecular breeding.

摘要

引言

WD40基因家族在真核生物中普遍存在，在细胞过程中发挥着多种作用。盐地碱蓬作为一种具有卓越耐盐性、耐涝性、繁殖和扩散能力的盐生植物，在工业应用和作物育种分析方面具有巨大潜力。探索该物种中与生长发育相关的基因，对于提高作物产量和环境适应性具有巨大潜力，尤其是在工业化种植园中。然而，我们对它们在调节植物生长发育中的作用的了解仍然有限。

方法

在本研究中，我们在全基因组水平上对盐地碱蓬中的WD40基因进行了全面分析，深入研究了它们的物理化学性质、系统发育关系、基因结构和表达模式等特征。此外，我们克隆了WD40基因，这是一个在不同物种的植物生长发育中都存在的基因。

结果

我们在盐地碱蓬基因组中总共鉴定出582个WD40蛋白，它们在染色体上分布不均。通过系统发育分析，我们将这582个盐地碱蓬WD40蛋白分为12个不同的进化枝。研究WD40基因的复制模式时，我们观察到片段重复在其扩增中起主要作用。相当一部分WD40基因复制对在进化过程中经历了纯化选择。为了探索其功能方面，我们选择了盐地碱蓬TTG1（SaTTG1）的同源物AtTTG1在拟南芥中进行过表达。亚细胞定位分析表明，SaTTG1蛋白定位于细胞核和质膜，在酵母细胞中表现出转录激活作用。AtTTG1在拟南芥中的过表达导致早花和种子大小增加。