Gene Bank, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru 502 324, Andhra Pradesh, India.
College of Agriculture, Anhui Science and Technology University, Fengyang, Anhui, China.
J Exp Bot. 2022 Jun 2;73(11):3584-3596. doi: 10.1093/jxb/erac110.
Sorghum is a feed/industrial crop in developed countries and a staple food elsewhere in the world. This study evaluated the sorghum mini core collection for days to 50% flowering (DF), biomass, plant height (PH), soluble solid content (SSC), and juice weight (JW), and the sorghum reference set for DF and PH, in 7-12 testing environments. We also performed genome-wide association mapping with 6 094 317 and 265 500 single nucleotide polymorphism markers in the mini core collection and the reference set, respectively. In the mini core panel we identified three quantitative trait loci for DF, two for JW, one for PH, and one for biomass. In the reference set panel we identified another quantitative trait locus for PH on chromosome 6 that was also associated with biomass, DF, JW, and SSC in the mini core panel. Transgenic studies of three genes selected from the locus revealed that Sobic.006G061100 (SbSNF4-2) increased biomass, SSC, JW, and PH when overexpressed in both sorghum and sugarcane, and delayed flowering in transgenic sorghum. SbSNF4-2 encodes a γ subunit of the evolutionarily conserved AMPK/SNF1/SnRK1 heterotrimeric complexes. SbSNF4-2 and its orthologs will be valuable in genetic enhancement of biomass and sugar yield in plants.
高粱在发达国家是一种饲料/工业作物，在世界其他地方则是主食。本研究评估了高粱微型核心种质库的抽穗至 50%开花日数 (DF)、生物量、株高 (PH)、可溶性固形物含量 (SSC) 和出汁量 (JW)，以及高粱参考系的 DF 和 PH，在 7-12 个测试环境中进行。我们还分别使用微型核心种质库和参考系中的 6094317 个和 265500 个单核苷酸多态性标记进行了全基因组关联图谱绘制。在微型核心群体中，我们鉴定出了 3 个与 DF 相关的数量性状位点，2 个与 JW 相关，1 个与 PH 相关，1 个与生物量相关。在参考系中，我们在 6 号染色体上又鉴定出了一个与 PH 相关的数量性状位点，该位点也与微型核心群体中的生物量、DF、JW 和 SSC 相关。从该位点选择的三个基因的转基因研究表明，Sobic.006G061100 (SbSNF4-2) 在高粱和甘蔗中过表达时会增加生物量、SSC、JW 和 PH，并使转基因高粱开花延迟。SbSNF4-2 编码进化上保守的 AMPK/SNF1/SnRK1 异三聚体复合物的 γ 亚基。SbSNF4-2 及其同源物将在提高植物的生物量和糖产量方面具有重要价值。
