Center of Excellence on Sustainable Disaster Management, Walailak University, Tha Sala, Nakon Sri Thammarat, 80161, Thailand.
Marine Institute, University of Plymouth, Drake Circus, Plymouth, PL4 8AA, UK.
Mar Environ Res. 2024 Jul;199:106566. doi: 10.1016/j.marenvres.2024.106566. Epub 2024 May 30.
The concentrations of Cd, Cu and Zn have been determined in the tissues and the cytosolic fraction of the common cockle, Cerastoderma edule, collected from sediments in the Tamar, Plym and Avon estuaries (South West, England). Metal concentrations in the tissues of C. edule from the Avon were lower than those from the Tamar and Plym, except for Cu in the digestive gland. Significant statistical relationships were only obtained between the total sedimentary metal concentrations and Cd in the body of C. edule and Cu in the digestive gland. The cytosolic fraction was extracted from each of the tissues and separated for protein analysis thereby allowing determination of the metal contents in high molecular weight (HMW) compounds, metallothionein-like proteins (MTLP) and very low molecular weight (VLMW) compounds. The digestive glands of C. edule from the Avon had relatively low concentrations of MTLP, whereas MTLP concentrations in the digestive gland of cockles from the Tamar and Plym were higher. The cytosolic fraction of C. edule had relatively low total Cd and Cu concentrations associated with MTLP, whereas Zn was preferentially associated with the HMW and the VLMW components. The results are relevant to metal distributions in C. edule and the role of cytosols in the management of metals by C. edule and other invertebrates.
从特马、普利茅斯和埃文河口（英格兰西南部）的沉积物中采集的普通贻贝（Cerastoderma edule）组织和胞浆部分的 Cd、Cu 和 Zn 浓度已经确定。来自埃文河口的 C. edule 的组织中的金属浓度低于来自特马和普利茅斯的金属浓度，除了消化腺中的 Cu 外。仅在 C. edule 体内的 Cd 和消化腺中的 Cu 与总沉积物金属浓度之间获得了显著的统计关系。从每个组织中提取胞浆部分并进行蛋白质分析，从而可以确定高分子量（HMW）化合物、金属硫蛋白样蛋白（MTLP）和极低分子量（VLMW）化合物中的金属含量。来自埃文河口的 C. edule 的消化腺中的 MTLP 浓度相对较低，而来自特马和普利茅斯贻贝的消化腺中的 MTLP 浓度较高。C. edule 的胞浆部分与 MTLP 相关的 Cd 和 Cu 总浓度相对较低，而 Zn 则优先与 HMW 和 VLMW 成分相关。研究结果与 C. edule 中的金属分布以及胞浆在 C. edule 和其他无脊椎动物对金属的管理中的作用有关。
