Coyote Bioscience (Beijing) Co., Ltd., Beijing, China.
Dr. Cuiyutao Healthcare Co., Ltd., Beijing, China.
Sci Rep. 2024 Jun 12;14(1):13516. doi: 10.1038/s41598-024-60683-6.
Diarrhea and constipation are common health concerns in children. Numerous studies have identified strong association between gut microbiota and digestive-related diseases. But little is known about the gut microbiota that simultaneously affects both diarrhea and constipation or their potential regulatory mechanisms. Stool samples from 618 children (66 diarrhea, 138 constipation, 414 healthy controls) aged 0-3 years were collected to investigate gut microbiota changes using 16S rRNA sequencing. Compared with healthy, children with diarrhea exhibited a significant decrease in microbial diversity, while those with constipation showed a marked increase (p < 0.05). Significantly, our results firstly Ruminococcus increased in constipation (p = 0.03) and decreased in diarrhea (p < 0.01) compared to healthy controls. Pathway analysis revealed that Ruminococcus highly involved in the regulation of five common pathways (membrane transport, nervous system, energy metabolism, signal transduction and endocrine system pathways) between diarrhea and constipation, suggesting a potential shared regulatory mechanism. Our finding firstly reveals one core microorganisms that may affect the steady balance of the gut in children with diarrhea or constipation, providing an important reference for potential diagnosis and treatment of constipation and diarrhea.
腹泻和便秘是儿童常见的健康问题。许多研究已经确定了肠道微生物群与消化相关疾病之间的强烈关联。但对于同时影响腹泻和便秘的肠道微生物群及其潜在的调节机制知之甚少。本研究收集了 618 名 0-3 岁儿童（66 名腹泻、138 名便秘、414 名健康对照）的粪便样本，通过 16S rRNA 测序来研究肠道微生物群的变化。与健康对照组相比，腹泻儿童的微生物多样性显著降低，而便秘儿童的微生物多样性显著增加（p < 0.05）。值得注意的是，与健康对照组相比，我们的结果首次发现 Ruminococcus 在便秘中增加（p = 0.03），在腹泻中减少（p < 0.01）。通路分析表明，Ruminococcus 高度参与了腹泻和便秘之间的五个常见通路（膜转运、神经系统、能量代谢、信号转导和内分泌系统通路）的调节，提示存在潜在的共同调节机制。本研究首次揭示了一种可能影响腹泻或便秘儿童肠道稳定平衡的核心微生物，为便秘和腹泻的潜在诊断和治疗提供了重要参考。
