Wasilauskas B, Gay R, Zwadyk P, Pfaller M, Koontz F
Department of Pathology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27103.
J Clin Microbiol. 1987 Dec;25(12):2355-8. doi: 10.1128/jcm.25.12.2355-2358.1987.
Recently, MicroScan (Baxter MicroScan Div., W. Sacramento, Calif.) introduced a radiolabeled-blood-culture system that is compatible with the BACTEC 460 (Johnston Laboratories, Inc., Towson, Md.). A multicenter blood culture study was initiated to evaluate this new system. Approximately 20 ml of blood was obtained from each patient and divided equally between BACTEC and MicroScan bottles which were incubated and processed identically. Aerobic bottles were examined twice on days 1 and 2 and once on days 3, 4, 5, 6, and 7. Anaerobic bottles were examined once a day for 7 days. There were 3,451 cultures evaluated, and 414 of these subsequently grew microorganisms. Of these positive cultures, 64 were judged to be contaminated. Of the remaining 350 positive cultures, 253 grew in both systems, 54 grew in BACTEC bottles only, and 43 grew in MicroScan bottles only. The average times to detect positive cultures were 1.8 and 2.1 days by the BACTEC and the MicroScan systems, respectively. No significant difference in the number or kind of organisms recovered or in the detection times for positive cultures was observed between the two blood-culturing systems.
最近，MicroScan（百特医疗MicroScan分部，加利福尼亚州西萨克拉门托）推出了一种与BACTEC 460（约翰斯顿实验室公司，马里兰州陶森）兼容的放射性标记血培养系统。启动了一项多中心血培养研究以评估这个新系统。从每位患者身上采集约20毫升血液，并在BACTEC瓶和MicroScan瓶之间平均分配，这些瓶子以相同方式进行培养和处理。需氧瓶在第1天和第2天检查两次，在第3、4、5、6和7天检查一次。厌氧瓶连续7天每天检查一次。共评估了3451份培养物，其中414份随后培养出微生物。在这些阳性培养物中，64份被判定为污染。在其余350份阳性培养物中，253份在两个系统中均生长，54份仅在BACTEC瓶中生长，43份仅在MicroScan瓶中生长。BACTEC系统和MicroScan系统检测到阳性培养物的平均时间分别为1.8天和2.1天。在两种血培养系统之间，所回收的微生物数量或种类以及阳性培养物的检测时间均未观察到显著差异。
