College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
China Resources Sanjiu (Huangshi) Pharmaceutical Co., Ltd., Huangshi, 435000, Hubei, China.
Sci Data. 2024 Jun 12;11(1):610. doi: 10.1038/s41597-024-03315-y.
Tinospora sagittata (Oliv.) Gagnep. is an important medicinal tetraploid plant in the Menispermaceae family. Its tuber, Radix Tinosporae, used in traditional Chinese medicine, is rich in diterpenoids and benzylisoquinoline alkaloids (BIAs). To enhance our understanding of medicinal compounds' biosynthesis and Menispermaceae's evolution, we herein report assembling a high-quality chromosome-scale genome with both PacBio HiFi and Illumina sequencing technologies. PacBio Sequel II generated 2.5 million circular consensus sequencing (CCS) reads, and a hybrid assembly strategy with Illumina sequencing resulted in 4483 contigs. The assembled genome size was 2.33 Gb, consisting of 4070 scaffolds (N50 = 42.06 Mb), of which 92.05% were assigned to 26 pseudochromosomes. T. sagittata's chromosomal-scale genome assembly, the first species in Menispermaceae, aids Menispermaceae evolution and T. sagittata's secondary metabolites biosynthesis understanding.
三叶崖爬藤（Oliv.）Gagnep. 是防己科中一种重要的四倍体药用植物。其块根，即钩藤，在中国传统医学中被广泛应用，富含二萜和苄基异喹啉生物碱（BIAs）。为了增进我们对药用化合物生物合成和防己科进化的理解，我们在此报告了利用 PacBio HiFi 和 Illumina 测序技术组装出高质量染色体水平基因组的工作。PacBio Sequel II 生成了 250 万个环状一致性测序（CCS）reads，而 Illumina 测序的混合组装策略则产生了 4483 个 contigs。组装的基因组大小为 2.33Gb，由 4070 个 scaffolds 组成（N50=42.06Mb），其中 92.05%被分配到 26 个假染色体上。三叶崖爬藤的染色体水平基因组组装是防己科中的第一个物种，有助于理解防己科的进化和三叶崖爬藤次生代谢产物的生物合成。
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