苦瓜基因组的染色体水平组装和进化分析。

Chromosome-level assembly and evolution analysis of the Trichosanthes truncata genome.

机构信息

National Center for Traditional Chinese Medicine Inheritance and Innovation, Guangxi Botanical Garden of Medicinal Plants, Nanning, 530023, China.

Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement, Guangxi Botanical Garden of Medicinal Plants, Nanning, 530023, China.

出版信息

Sci Data. 2024 Aug 12;11(1):872. doi: 10.1038/s41597-024-03608-2.

DOI:10.1038/s41597-024-03608-2

PMID:39134552

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11319624/

Abstract

Trichosanthes truncata C. B. Clarke, an important medicinal plant, is a dioecious plant belonging to the Cucurbitaceae family. This study presents a chromosomal-level reference genome assembly for T. truncata. Through the integration of PacBio high-fidelity sequencing and high-throughput chromosome conformation capture technology, a final genome sequence of 637.41 Mb was assembled, with an N50 of 57.24 Mb and consisting of 11 pseudochromosomes. Additionally, 97.21 Mb of repetitive sequences and 36,172 protein-coding genes were annotated. This high-quality genome assembly is of utmost significance for studying the molecular mechanisms underlying the biosynthesis of bioactive compounds. Furthermore, this study provided valuable insights into plant comparative genomics research.

摘要

栝楼，一种重要的药用植物，是葫芦科的雌雄异株植物。本研究提供了一个栝楼的染色体水平的参考基因组组装。通过 PacBio 高保真测序和高通量染色体构象捕获技术的整合，最终组装出 637.41 Mb 的基因组序列，N50 为 57.24 Mb，由 11 条假染色体组成。此外，还注释了 97.21 Mb 的重复序列和 36172 个蛋白质编码基因。这个高质量的基因组组装对于研究生物活性化合物生物合成的分子机制具有重要意义。此外，本研究为植物比较基因组学研究提供了有价值的见解。