Sociosexuality refers to the tendency to engage in uncommitted sexual behavior and has been dissected into three domains: sociosexual behavior, attitudes, and desire (Penke & Asendorpf, 2008), which led to the revised Sociosexual Orientation Inventory (SOI-R), which was validated on a German sample. The current research aimed at translating and validating an Italian version (I-SOI-R), administered to three distinct Italian participant groups. In the first sample (N = 710, females = 521, age = 18-59 years), we found evidence for a bifactor model, articulated in a general sociosexuality factor and three specific factors (behavior, attitudes, desire). High internal consistency was established for total and subscale scores, alongside favorable test-retest reliability. A connection was found between relationship status and sociosexual desire, though not gender dependent. We found evidence for test-retest reliability in a second sample (N = 55, females = 37, age 20-58 years). In a third study (N = 305, females = 147, age = 19-60 years), the earlier findings were replicated, further confirming the I-SOI-R's construct, criterion, and nomological validity on an online sample. Combining data from the three studies revealed full configural, metric, and scalar invariance regarding gender. This allowed us to meaningfully compare the observed scores of women and men and replicated the finding that men display higher levels of unrestricted sociosexuality. In conclusion, the I-SOI-R may serve as a valuable tool to assess and enhance sexual health, albeit warranting future research on construct and criterion validity.