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一种用于宏观和形态测量检查(尤其是人脑检查)的新的包埋和切片技术(振动切片机)。

A new embedding and sectioning technique (macrovibratome) for macroscopic and morphometric examinations especially of the human brain.

作者信息

Klekamp J, Riedel A, Herrmann A, Kretschmann H J

出版信息

J Hirnforsch. 1985;26(1):33-40.

PMID:3886780
Abstract

The macrovibratome is a newly developed sectioning apparatus designed especially for the preparation of human brain material subject to macroscopic and morphometric examinations. The brain is embedded in an aqueous agar solution and cut into slices with a minimum thickness of 2 mm. Using a 20% or 40% gelatin solution as the embedding material results in an average shrinkage of the tissue blocks of 9.8 or 31.7% in volume, respectively. With a 3% agar solution the volume shrinkage lies on the average at 1.0% for smaller brain pieces and 4.5% for cerebra.

摘要

大型振动切片机是一种新开发的切片设备,专门设计用于制备用于宏观和形态计量学检查的人脑材料。将大脑嵌入水琼脂溶液中,并切成最小厚度为2毫米的切片。使用20%或40%的明胶溶液作为包埋材料时,组织块的体积平均分别收缩9.8%或31.7%。使用3%的琼脂溶液时,较小脑块的体积平均收缩率为1.0%,大脑的平均收缩率为4.5%。

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