Crecelius Anna C, Cornett D Shannon, Caprioli Richard M, Williams Betsy, Dawant Benoit M, Bodenheimer Bobby
Mass Spectrometry Research Center and Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37232, USA.
J Am Soc Mass Spectrom. 2005 Jul;16(7):1093-9. doi: 10.1016/j.jasms.2005.02.026.
We have developed a method to visualize matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI IMS) data aligned with optically determinable tissue structures in three dimensions. Details of the methodology are exemplified using the 3-D reconstruction of myelin basic protein (MBP) in the corpus callosum of a mouse brain. In this procedure, optical images obtained from serial coronal sections are first aligned to each other to reconstruct a surface of the corpus callosum from segmented contours of the aligned images. The MALDI IMS data are then coregistered to the optical images and superimposed into the surface to create the final 3-D visualization. Correlating proteomic data with anatomical structures provides a more comprehensive understanding of healthy and pathological brain functions, and holds promise to be utilized in more complex anatomical arrangements.
我们开发了一种方法，用于可视化与三维光学可确定组织结构对齐的基质辅助激光解吸电离成像质谱（MALDI IMS）数据。使用小鼠脑胼胝体中髓鞘碱性蛋白（MBP）的三维重建对该方法的细节进行了举例说明。在这个过程中，首先将从连续冠状切片获得的光学图像相互对齐，以从对齐图像的分割轮廓重建胼胝体的表面。然后将MALDI IMS数据与光学图像进行配准，并叠加到表面上以创建最终的三维可视化。将蛋白质组学数据与解剖结构相关联，能够更全面地理解健康和病理状态下的脑功能，并且有望应用于更复杂的解剖结构。
