Seevalingam Rajeletchmi, Yahaya Norziha, Syed Mohamed Alizae Marny F, Kumar Hetal A
Department of Restorative Dentistry, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur, MYS.
Department of Family Oral Health, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur, MYS.
Cureus. 2024 May 13;16(5):e60226. doi: 10.7759/cureus.60226. eCollection 2024 May.
Protocols that determine the lesion depth of specific demineralization solutions are lacking. This in vitro study aimed to evaluate various lesion depths of artificial white spot lesions (WSLs) at different exposure times. Materials and methods: Artificial WSLs were created by placing 30 extracted human premolar teeth into 0.05M acetate buffer solution with a controlled environment of pH 4.4 at 37ºC, which were then exposed in the solution for various durations of 4, 5, 6, 8, 10, and 12 days. The specimens were visually examined using the Ekstrand-Ricketts-Kidd (ERK) system to confirm the WSL, followed by buccolingual sectioning, and evaluated under a scanning electron microscope (SEM) to measure the lesion depth.
The SEM showed that the mean lesion depths of representative specimens were 101.33 µm (day 4), 124 µm (day 5), 159 µm (day 6), 187 µm (day 8), 202 µm (day 10), and 212 µm (day 12). The artificial WSL was visually demonstrated in grades 1 and 2 of the ERK system.
The depths of the lesions increased as the duration increased from day 4 to day 12, indicating that the lesion depths increased with the more prolonged exposure to the acetate buffer solution.
目前缺乏确定特定脱矿溶液病变深度的方案。本体外研究旨在评估人工白斑病变（WSLs）在不同暴露时间下的各种病变深度。
将30颗拔除的人类前磨牙置于pH值为4.4、温度为37ºC的0.05M醋酸缓冲溶液中，在可控环境下制造人工WSLs，然后将其在该溶液中暴露4、5、6、8、10和12天等不同时长。使用埃克斯特兰德-里基茨-基德（ERK）系统对标本进行目视检查以确认WSL，随后进行颊舌向切片，并在扫描电子显微镜（SEM）下评估以测量病变深度。
扫描电子显微镜显示，代表性标本的平均病变深度分别为101.33 µm（第4天）、124 µm（第5天）、159 µm（第6天）、187 µm（第8天）、202 µm（第10天）和212 µm（第12天）。人工WSL在ERK系统的1级和2级中得到目视证实。
从第4天到第12天，随着暴露时间的增加，病变深度增加，这表明随着在醋酸缓冲溶液中暴露时间的延长，病变深度增加。
