Tavassoli Maryam, Kadivar Mehdi, Akhavan Amir Ahmad, Abdigoudarzi Mohammad, Moridnia Abbas, Chaibakhsh Samira, Beikmohammadi Mojtaba, Mehdi Sedaghat Mohammad
Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran.
J Arthropod Borne Dis. 2023 Dec 31;17(4):352-363. doi: 10.18502/jad.v17i4.15298. eCollection 2023 Dec.
The saliva and salivary glands of ticks possess a wide range of immuno-pharmacologically active molecules that effectively modulate the activity of enzymes, antibodies, and amines that have a role in different biological processes. Derived components from saliva and salivary glands of hard ticks Ixodidae have been characterized as potential natural sources for discovering promising anti-cancer drug candidates.
The anti-cancer activity of salivary gland extracts (SGEs) from , , , and was assessed. MTT assays and flow cytometry were done on the HT-29 colorectal cancer cell line to evaluate the anti-viability and proliferative inhibition.
Based on the MTT assay results, the SGEs from had the highest and lowest substantial anti-viability effects on the HT-29 cancer cell and human foreskin fibroblast (HFF) normal cell, respectively. The cytometric assessment revealed a significant increase in the apoptosis and necrosis ratio of the HT-29 cancer cells after treatment with SGEs.
The results demonstrated that SGEs have significant anti-proliferative, anti-viability, and apoptotic potential. The result of this study suggests that SGEs is an appropriate candidate for further investigations to identify and purify the mechanisms and molecules involved in the anti-cancer activity of the SGEs.
蜱虫的唾液和唾液腺含有多种免疫药理活性分子，可有效调节在不同生物过程中起作用的酶、抗体和胺的活性。硬蜱科蜱虫唾液和唾液腺的衍生成分已被鉴定为发现有前景的抗癌药物候选物的潜在天然来源。
评估了来自[具体蜱虫种类1]、[具体蜱虫种类2]、[具体蜱虫种类3]和[具体蜱虫种类4]的唾液腺提取物（SGEs）的抗癌活性。对HT - 29结肠癌细胞系进行MTT试验和流式细胞术，以评估抗生存能力和增殖抑制情况。
基于MTT试验结果，来自[具体蜱虫种类1]的SGEs对HT - 29癌细胞和人包皮成纤维细胞（HFF）正常细胞分别具有最高和最低的显著抗生存能力作用。细胞计量评估显示，用[具体蜱虫种类1]的SGEs处理后，HT - 29癌细胞的凋亡和坏死率显著增加。
结果表明[具体蜱虫种类1]的SGEs具有显著的抗增殖、抗生存能力和凋亡潜力。本研究结果表明，[具体蜱虫种类1]的SGEs是进一步研究以鉴定和纯化参与SGEs抗癌活性的机制和分子的合适候选物。
