Lamster I B, Vogel R I, Hartley L J, DeGeorge C A, Gordon J M
J Periodontol. 1985 Mar;56(3):139-47. doi: 10.1902/jop.1985.56.3.139.
Experimental gingivitis provides a useful model for studying the initiation of periodontal disease in man. This study evaluated over a 4-week period the Plaque Index (PLI), Gingival Bleeding Time Index (GBTI), and gingival crevicular fluid (GCF) for resting and flow volume as well as the concentration and total activity of three enzymes in the GCF (lactate dehydrogenase--LDH, beta-glucuronidase--BG and arylsulfatase--AS) from the maxillary right quadrant of eight subjects with healthy gingiva. After rising sharply during the 1st week, the PLI continued to increase during the 2nd week but remained constant during the 3rd and 4th weeks. The GBTI, and the resting and flow GCF volumes, increased steadily throughout the study. LDH concentration in GCF varied minimally during the experiment, while total LDH activity rose slightly over the 4-week period. BG concentration and total activity in GCF rose steadily from baseline to the 3rd week and then either fell or leveled off during the 4th week. AS concentration in GCF rose from baseline to the 2nd or 3rd week and then fell. AS total activity in GCF rose from baseline to the 2nd week and then remained constant. These data suggest that while clinical signs of inflammation increased over the 4 weeks of the experiment, a homeostatic mechanism in the crevicular environment may control ground substance-degrading enzyme activity during experimental gingivitis in man.
实验性牙龈炎为研究人类牙周疾病的发病机制提供了一个有用的模型。本研究在4周的时间内，对8名牙龈健康受试者上颌右象限的菌斑指数（PLI）、牙龈出血时间指数（GBTI）、龈沟液（GCF）的静息量和流量以及GCF中三种酶（乳酸脱氢酶-LDH、β-葡萄糖醛酸酶-BG和芳基硫酸酯酶-AS）的浓度和总活性进行了评估。在第1周急剧上升后，PLI在第2周继续增加，但在第3周和第4周保持稳定。在整个研究过程中，GBTI以及GCF的静息量和流量稳步增加。实验期间，GCF中LDH的浓度变化最小，而LDH的总活性在4周内略有上升。GCF中BG的浓度和总活性从基线稳步上升至第3周，然后在第4周下降或趋于平稳。GCF中AS的浓度从基线上升至第2周或第3周，然后下降。GCF中AS的总活性从基线上升至第2周，然后保持稳定。这些数据表明，虽然在实验的4周内炎症的临床体征有所增加，但龈沟环境中的一种稳态机制可能在人类实验性牙龈炎期间控制细胞外基质降解酶的活性。
